anti-Rat CKMT2 Monoclonal Antibody | anti-CKMT2 antibody

Anti-CKMT2 Mouse mAb

Cat. Num. AAA475022

• Gene Names: CKMT2; SMTCK
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: CKMT2; Monoclonal Antibody; Anti-CKMT2 Mouse mAb; CKMT 2; Basic-type mitochondrial creatine kinase; CPK; Creatine kinase mitochondrial 2; Creatine kinase mitochondrial 2 (sarcomeric); Creatine kinase S-type; creatine kinase S-type; mitochondrial; KCRS_HUMAN; Mib CK; Mib-CK; OTTHUMP00000147542; S-MtCK; Sarcomeric mitochondrial creatine kinase; SMTCK; anti-CKMT2 antibody

• Host: Mouse; Source: Human
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3F4-G5-H5
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%, glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 419

• Applicable Applications for anti-CKMT2 antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: Purified recombinant human CKMT2 protein fragments expressed in E Coli.
• Sensitivity: This antibody detects endogenous levels of CKMT2 and does not cross-react with related proteins.
• Sequence Similarities: Belongs to the ATP:guanido phosphotransferase family. Contains 1 phosphagen kinase C-terminal domain. Contains 1 phosphagen kinase N-terminal domain.
• Subunit Structure: Exists as an octamer composed of four CKMT2 homodimers.
• Subcellular Location: Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Western Blot (WB)

(Western blot detection of CKMT2 in Rat Brain lysates using CKMT2 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.)

Related Product Information for anti-CKMT2 antibody

Entrez Summary: Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.

Product Categories/Family for anti-CKMT2 antibody

Metabolism; Pathways and Processes; Metabolic signaling pathways; Energy transfer pathways; Energy Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 153251984
• NCBI GeneID: 1160
• NCBI Accession #: NP_001093205.1
• NCBI GenBank Nucleotide #: NM_001825.2
• UniProt Accession #: P17540; Q6ICS8; Q8N1E1
• Molecular Weight: 47
• NCBI Official Full Name: creatine kinase S-type, mitochondrial
• NCBI Official Synonym Full Names: creatine kinase, mitochondrial 2
• NCBI Official Symbol: CKMT2
• NCBI Official Synonym Symbols: SMTCK
• NCBI Protein Information: creatine kinase S-type, mitochondrial
• UniProt Protein Name: Creatine kinase S-type, mitochondrial
• Protein Family: Creatine kinase
• UniProt Gene Name: CKMT2
• UniProt Synonym Gene Names: Mib-CK; S-MtCK

NCBI Description

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CKMT2: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family.

Protein type: Amino Acid Metabolism - arginine and proline; EC 2.7.3.2; Kinase, other; Mitochondrial

Chromosomal Location of Human Ortholog: 5q14.1

Cellular Component: mitochondrial inner membrane; mitochondrion

Molecular Function: creatine kinase activity

Biological Process: creatine metabolic process; muscle contraction