Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human carcinoid stained with MBS602178 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.)
Mouse Chromogranin A, mixed clones Monoclonal Antibody
Chromogranin A, mixed clones (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI)
Reactivity
Human, Monkey
Applications
Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Chromogranin A; mixed clones; Monoclonal Antibody; mixed clones (BSA & Azide Free) (CGA; CHG A; Pancreastatin; Parathyroid secretory protein 1; Pituitary secretory protein I; Vasostatin; SPI); Anti -Chromogranin A; anti-Chromogranin A; mixed clones antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F131
Specificity
The cocktail is especially designed for sensitive detection of Chromogranin A in formalin-fixed, paraffin-embedded tissues. Species Crossreactivity: human and monkey.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide.
Applicable Applications for anti-Chromogranin A, mixed clones antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry (formalin/paraffin).
Dilution: Immunohistochemistry: 0.5-1.0ug/ml for 30 min at RT. Although not required, staining of formalin/paraffin tissues is ENHANCED by boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Dilution: Immunohistochemistry: 0.5-1.0ug/ml for 30 min at RT. Although not required, staining of formalin/paraffin tissues is ENHANCED by boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Optimal dilution determined by the researcher.
Immunogen
Human pheochromocytoma
Cellular Localization
Cytoplasmic
Positive Control
Pancreas
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at-20 degree C or colder. Aliquots are stable for at least 12 months at-20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human carcinoid stained with MBS602178 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human carcinoid stained with MBS602178 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of tumor cells.)
Related Product Information for anti-Chromogranin A, mixed clones antibody
Chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Product Categories/Family for anti-Chromogranin A, mixed clones antibody
Similar Products
Product Notes
The Chromogranin A, mixed clones (Catalog #AAA602178) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Chromogranin A, mixed clones (BSA & Azide Free) (CGA, CHG A, Pancreastatin, Parathyroid secretory protein 1, Pituitary secretory protein I, Vasostatin, SPI) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Chromogranin A, mixed clones can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry (formalin/paraffin). Dilution: Immunohistochemistry: 0.5-1.0ug/ml for 30 min at RT. Although not required, staining of formalin/paraffin tissues is ENHANCED by boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the Chromogranin A, mixed clones for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chromogranin A, mixed clones, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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