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SDS-Page

Dual Serine/Threonine And Tyrosine Protein Kinase Recombinant Protein | DSTYK recombinant protein

Recombinant Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK)

Gene Names
Dstyk; Ripk5; DustyPK
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
>90%
Synonyms
Dual Serine/Threonine And Tyrosine Protein Kinase; Recombinant Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK); DSTYK recombinant protein
Ordering
For Research Use Only!
Host
Host: E Coli
Source: Prokaryotic expression
Purity/Purification
>90%
Form/Format
Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
P KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMRSLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLSLETRLQIALDVV EGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPEAMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC SGSIKLPEAFERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PSKRPLLGIVQPIL
Sequence Length
927
Applicable Applications for DSTYK recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Predicted Molecular Mass
32.6kDa
Accurate Molecular Mass (KD)
33kDa
Endotoxin
<1.0EU per 1ug (determined by the LAL method)
Expression System
Prokaryotic expression
Tag
two N-terminal Tags, His-tag and S-tag
Organism Species
Rattus norvegicus (Rat)
Fragment
Pro650~Leu904
Usage
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
104,898 Da
NCBI Official Full Name
dual serine/threonine and tyrosine protein kinase
NCBI Official Synonym Full Names
dual serine/threonine and tyrosine protein kinase
NCBI Official Symbol
Dstyk
NCBI Official Synonym Symbols
Ripk5; DustyPK
NCBI Protein Information
dual serine/threonine and tyrosine protein kinase
UniProt Protein Name
Dual serine/threonine and tyrosine protein kinase
UniProt Gene Name
Dstyk
UniProt Synonym Gene Names
Ripk5; Dusty PK
UniProt Entry Name
DUSTY_RAT

NCBI Description

human homolog is a RIP-related serine/threonine protein kinase which can induce apoptosis through both caspase-dependent and -independent pathways [RGD, Feb 2006]

Uniprot Description

Acts as a positive regulator of ERK phosphorylation downstream of fibroblast growth factor-receptor activation. May induce both caspase-dependent apoptosis and caspase-independent cell death.

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Product Notes

The DSTYK dstyk (Catalog #AAA2029618) is a Recombinant Protein produced from Host: E Coli Source: Prokaryotic expression and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Dual Serine/Threonine And Tyrosine Protein Kinase can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the DSTYK dstyk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
P KLGQELGRGQ YGVVYLCDNW GGHFPCALKS VVPPDEKHWN DLALEFHYMR SLPKHERLVD LHGSVIDYNY GGGSSVAVLL IMERLHRDLY TGLKAGLSLE TRLQIALDVV EGIRFLHSQG LVHRDIKLKN VLLDKQNRAK ITDLGFCKPE AMMSGSIVGT PIHMAPELFT GKYDNSVDVY AFGILFWYIC SGSIKLPEAF ERCASKDHLW NNVRRGTRPE RLPVFDEECW QLMEACWDGD PSKRPLLGIV QPIL. It is sometimes possible for the material contained within the vial of "Dual Serine/Threonine And Tyrosine Protein Kinase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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