Mouse CHOP Monoclonal Antibody | anti-CHOP antibody

CHOP Monoclonal Antibody

Cat. Num. AAA2548916

• Gene Names: DDIT3; CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Protein A Purification
• Synonyms: CHOP; Monoclonal Antibody; CHOP Monoclonal Antibody; DDIT3; anti-CHOP antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4C3
• Purity/Purification: Protein A Purification
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 192

• Applicable Applications for anti-CHOP antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC: 1:100-1:200; IF: 1:100-1:300
• Immunogen: Synthetic Peptide of CHOP
• Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of Mouse liver using CHOP Monoclonal Antibody at dilution of 1:2000.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human stomach carcinoma tissue using CHOP Monoclonal Antibody at dilution of 1:200.)

Immunofluorescence (IF)

(Immunofluorescence analysis of Mouse brain tissue using CHOP Monoclonal Antibody at dilution of 1:200.)

Related Product Information for anti-CHOP antibody

Multifunctional transcription factor in ER stress response. Plays an essential role in the response to a wide variety of cell stresses and induces cell cycle arrest and apoptosis in response to ER stress. Plays a dual role both as an inhibitor of CCAAT/enhancer-binding protein (C/EBP) function and as an activator of other genes. Acts as a dominant-negative regulator of C/EBP-induced transcription: dimerizes with members of the C/EBP family, impairs their association with C/EBP binding sites in the promoter regions, and inhibits the expression of C/EBP regulated genes. Positively regulates the transcription of TRIB3, IL6, IL8, IL23, TNFRSF10B/DR5, PPP1R15A/GADD34, BBC3/PUMA, BCL2L11/BIM and ERO1L. Negatively regulates; expression of BCL2 and MYOD1, ATF4-dependent transcriptional activation of asparagine synthetase (ASNS), CEBPA-dependent transcriptional activation of hepcidin (HAMP) and CEBPB-mediated expression of peroxisome proliferator-activated receptor gamma (PPARG). Inhibits the canonical Wnt signaling pathway by binding to TCF7L2/TCF4, impairing its DNA-binding properties and repressing its transcriptional activity. Plays a regulatory role in the inflammatory response through the induction of caspase-11 (CASP4/CASP11) which induces the activation of caspase-1 (CASP1) and both these caspases increase the activation of pro-IL1B to mature IL1B which is involved in the inflammatory response.

Product Categories/Family for anti-CHOP antibody

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 304282225
• NCBI GeneID: 1649
• NCBI Accession #: NP_001181982.1
• NCBI GenBank Nucleotide #: NM_001195053.1
• UniProt Accession #: P35638; F8VS99
• Molecular Weight: 27kDa
• NCBI Official Full Name: DNA damage-inducible transcript 3 protein isoform 1
• NCBI Official Synonym Full Names: DNA damage inducible transcript 3
• NCBI Official Symbol: DDIT3
• NCBI Official Synonym Symbols: CHOP; CEBPZ; CHOP10; CHOP-10; GADD153; C/EBPzeta
• NCBI Protein Information: DNA damage-inducible transcript 3 protein
• UniProt Protein Name: DNA damage-inducible transcript 3 protein
• UniProt Gene Name: DDIT3
• UniProt Synonym Gene Names: CHOP; CHOP10; GADD153; DDIT-3; CHOP; CHOP-10

NCBI Description

This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010]

Uniprot Description

DDIT3: a transcriptional-regulatory protein of the bZIP family. Inhibits the DNA-binding activity of C/EBP and LAP by forming heterodimers that cannot bind DNA. May play an important role in melanoma progression. CK2-mediated phosphorylation inhibits its transcriptional activity. Up-regulates IL-6 transcription by trapping negative regulating NF-IL6 isoform.

Protein type: Autophagy; DNA-binding; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 12q13.3

Cellular Component: CHOP-ATF3 complex; CHOP-ATF4 complex; CHOP-C/EBP complex; cytosol; late endosome; nucleoplasm; nucleus; transcription factor AP-1 complex

Molecular Function: cAMP response element binding protein binding; DNA binding; DNA binding transcription factor activity; leucine zipper domain binding; protein binding; protein heterodimerization activity; protein homodimerization activity; transcription corepressor activity; transcription factor binding; transcription regulatory region DNA binding

Biological Process: ATF6-mediated unfolded protein response; blood vessel maturation; cell cycle arrest; cell redox homeostasis; cellular response to DNA damage stimulus; ER overload response; establishment of protein localization to mitochondrion; inhibition of CREB transcription factor; intrinsic apoptotic signaling pathway in response to nitrosative stress; mRNA transcription from RNA polymerase II promoter; negative regulation of canonical Wnt signaling pathway; negative regulation of determination of dorsal identity; negative regulation of fat cell differentiation; negative regulation of myoblast differentiation; negative regulation of protein kinase B signaling cascade; negative regulation of transcription factor activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; PERK-mediated unfolded protein response; positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; positive regulation of interleukin-8 production; positive regulation of neuron apoptotic process; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress; positive regulation of transcription, DNA-templated; proteasomal ubiquitin-dependent protein catabolic process; protein oligomerization; regulation of DNA-templated transcription in response to stress; regulation of transcription involved in anterior/posterior axis specification; regulation of transcription, DNA-templated; release of sequestered calcium ion into cytosol; response to starvation; response to unfolded protein; Wnt signaling pathway

Disease: Myxoid Liposarcoma