Mouse Chondroitin Sulphate Neoepitope Monoclonal Antibody
Monocloncal Antibody to Chondroitin Sulphate Neoepitope Antibody (clone 1B5)
Applications
Western Blot, Immunohistochemistry
Purity
Protein A
Synonyms
Chondroitin Sulphate Neoepitope; Monoclonal Antibody; Monocloncal Antibody to Chondroitin Sulphate Neoepitope Antibody (clone 1B5); Chondroitin Sulphate Neoepitope Antibody; anti-Chondroitin Sulphate Neoepitope antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1K
Clone Number
1B5
Specificity
This antibody recognizes zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Purity/Purification
Protein A
Form/Format
Clear liquid
Concentration
0.1 mg/mL (varies by lot)
Applicable Applications for anti-Chondroitin Sulphate Neoepitope antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot: use at dilution of 1/100.; Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections.
Immunogen
Bovine nasal cartilage aggrecan that had been deglycosylated with chondroitinase ABC digestion; i.e. BNC (ABC) Core (see Couchman et al 1984 and Caterson et al 1985).
Buffer
PBS, no preservatives
Technical Notes
Samples are usually deglycosylated using 0.01 Units Chondroitinase ABC (Sigma), 0.01 Units Keratanase (Seikagaku) and 0.0001 Units Keratanase II (Seikagaku) per 10ug S-GAG of non-deglycosylated aggrecan for optimal epitope recognition in SDS-PAGE and immunohistochemistry.
Preparation and Storage
Store at -20 degree C. Avoid repeating freezing and thawing. Aliquoting is recommended.
Related Product Information for anti-Chondroitin Sulphate Neoepitope antibody
Chondroitin sulfate monoclonal antibody (mouse, clone 1B5) to detect the zero sulphated Chondroitin Sulphate stub neoepitope generated by chondroitinase ABC treatment.
Overview: Recognizes delta unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.
Overview: Recognizes delta unsaturated disaccharide of unsulfated chondroitin generated by chondroitinase ABC digestion.
Product Categories/Family for anti-Chondroitin Sulphate Neoepitope antibody
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Product Notes
The Chondroitin Sulphate Neoepitope (Catalog #AAA442014) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Chondroitin Sulphate Neoepitope can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot: use at dilution of 1/100.; Immunohistochemistry: Formalin or paraformaldehyde-fixed paraffin embedded sections, alcohol-fixed frozen sections or un-fixed snap-frozen sections. Researchers should empirically determine the suitability of the Chondroitin Sulphate Neoepitope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chondroitin Sulphate Neoepitope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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