Mouse Chloramphenicol Monoclonal Antibody
Chloramphenicol (Chloromycetin) (Biotin)
Applications
ELISA
Purity
Purified by Protein G Affinity Chromatography. >=95% by SDS-PAGE.
Synonyms
Chloramphenicol; Monoclonal Antibody; Chloramphenicol (Chloromycetin) (Biotin); anti-Chloramphenicol antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10i3
Specificity
Recognizes chloramphenicol.
Purity/Purification
Purified by Protein G Affinity Chromatography. >=95% by SDS-PAGE.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Labeled with Biotin.
Applicable Applications for anti-Chloramphenicol antibody
ELISA (EIA)
Application Notes
Suitable for use in competitive ELISA.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Chloramphenicol-BSA conjugate
Conjugate
Biotin
Hybridoma
Sp2/0 myeloma cells with spleen cells from BALB/c mice.
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Chloramphenicol antibody
Chloramphenicol is a chlorine-containing antibiotic produced by Streptomyces venezuelae. It has a wide spectrum of activity against gram-positive and gram-negative cocci and bacilli (including anaerobes), Rickettsia, Mycoplasma and Chlamydia. It inhibits prokaryotic protein synthesis by attaching to the 50S ribosomal subunit. This inhibits peptidyltransferase, thereby preventing the formation of peptide bonds. It also inhibits protein synthesis in mitochondria which accounts for its toxic effects that cause aplastic anemia. Chloramphenicol has other adverse effects such as bone marrow depression and gray baby syndrome. It's use is limited to serious infections such as those where resistance to other antibiotics occurs. It is still widely used in the treatment of typhoid fever, meningitis and eye infections.
Product Categories/Family for anti-Chloramphenicol antibody
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Product Notes
The Chloramphenicol (Catalog #AAA6127322) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Chloramphenicol can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Suitable for use in competitive ELISA. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Chloramphenicol for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Chloramphenicol, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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