Mouse anti-Human CELF1 Monoclonal Antibody | anti-CELF1 antibody

CELF1 Monoclonal Antibody

Cat. Num. AAA3014459

• Gene Names: CELF1; CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Protein affinity purified.
• Synonyms: CELF1; Monoclonal Antibody; CELF1 Monoclonal Antibody; CELF-1; CUG-BP1; EDEN-BP homolog; BRUNOL2; CUGBP; CUGBP1; NAB50; 50 kDa Nuclear polyadenylated RNA binding protein; 50 kDa nuclear polyadenylated RNA-binding protein; Bruno like 2; bruno like protein 2; Bruno-like protein 2; BRUNOL 2; CELF 1; celf1; CELF1 CUGBP; Elav like family member 1; CUG BP and ETR 3 like factor 1; CUG BP; CUG BP1; CUG RNA binding protein; CUG triplet repeat RNA binding protein 1; CUG triplet repeat RNA-binding protein 1; CUG-BP; CUG-BP- and ETR-3-like factor 1; CUGBP 1; CUGBP and ETR3 like factor 1; CUGBP Elav like family member 1; CUGBP Elav-like family member 1; Cytidine uridine guanosine binding protein 1; Deadenylation factor CUG BP; Deadenylation factor CUG-BP; Deadenylation factor CUGBP; EDEN BP; EDEN BP homolog; EDEN-BP; embryo deadenylation element binding protein; embryo deadenylation element binding protein homolog; Embryo deadenylation element-binding protein homolog; hNab 50; hNab50; NAB 50; NAPOR; Nuclear polyadenylated RNA binding protein 50 kD; Nuclear polyadenylated RNA binding protein; RNA binding protein BRUNOL 2; RNA binding protein BRUNOL2; RNA-binding protein BRUNOL-2; anti-CELF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: CELF1 monoclonal antibody detects endogenous levels of CELF1 protein.
• Purity/Purification: Protein affinity purified.
• Form/Format: Mouse IgG1, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 486

• Applicable Applications for anti-CELF1 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:2,000-1:5,000
• Immunogen: Recombinant protein within human CELF1 aa 1-180.
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of CELF1 on Daudi cell lysate using anti-CELF1 antibody at 1/5,000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CELF1 antibody. Counter stained with hematoxylin.)

Related Product Information for anti-CELF1 antibody

Myotonic dystrophy (DM) is an autosomal dominant neuromuscular disease that is associated with a (CTG)n repeat expansion in the 3'-untranslated region of the myotonin protein kinase gene (DMPK). CUG-BP1 and CUG-BP2 are proteins that bind specifically to (CUG)8 oligonucleotides in vitro. While CUG-BP1 has the major binding activity in normal cells, nuclear CUG-BP2 binding activity increases in DM cells. Both CUG-BP1 and CUG-BP2 are isoforms of a novel heterogeneous nuclear ribonucleoprotein (hnRNP), hNab50. CUG-BP1, an RNA CUG triplet repeat binding protein, regulates splicing and translation of various RNAs. Expansion of RNA CUG repeats in the DMPK in DM is associated with alterations in binding activity of CUG-BP1 as well as alterations in the translation of the C/EBPb transcription factor. CUG-BP1 is an important regulator of initiation from different AUG codons of C/EBPb mRNA. In normal cells, CUG-BP1 up-regulates the p21 protein during differentiation by inducing the translation of p21 via binding to a GC-rich sequence located within the 5' region of p21 mRNA. In DM cells, failure to accumulate CUG-BP1 leads to a reduction of p21 and alterations in other proteins responsible for cell cycle withdrawl.

NCBI and Uniprot Product Information

• NCBI GI #: 71164871
• NCBI GeneID: 10658
• NCBI Accession #: NP_001020767.1
• NCBI GenBank Nucleotide #: NP_001020767.1
• UniProt Accession #: Q92879
• Molecular Weight: 52kDa
• NCBI Official Full Name: CUGBP Elav-like family member 1 isoform 3
• NCBI Official Synonym Full Names: CUGBP Elav-like family member 1
• NCBI Official Symbol: CELF1
• NCBI Official Synonym Symbols: CUGBP; NAB50; NAPOR; CUG-BP; CUGBP1; hNab50; BRUNOL2; EDEN-BP
• NCBI Protein Information: CUGBP Elav-like family member 1
• UniProt Protein Name: CUGBP Elav-like family member 1
• Protein Family: CUGBP Elav-like family
• UniProt Gene Name: CELF1
• UniProt Synonym Gene Names: BRUNOL2; CUGBP; CUGBP1; NAB50; CELF-1; CUG-BP1
• UniProt Entry Name: CELF1_HUMAN

NCBI Description

Members of the CELF/BRUNOL protein family contain two N-terminal RNA recognition motif (RRM) domains, one C-terminal RRM domain, and a divergent segment of 160-230 aa between the second and third RRM domains. Members of this protein family regulate pre-mRNA alternative splicing and may also be involved in mRNA editing, and translation. This gene may play a role in myotonic dystrophy type 1 (DM1) via interactions with the dystrophia myotonica-protein kinase (DMPK) gene. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CELF1: RNA-binding protein implicated in the regulation of several post-transcriptional events. Involved in pre-mRNA alternative splicing, mRNA translation and stability. Mediates exon inclusion and/or exclusion in pre-mRNA that are subject to tissue-specific and developmentally regulated alternative splicing. Specifically activates exon 5 inclusion of cardiac isoforms of TNNT2 during heart remodeling at the juvenile to adult transition. Acts as both an activator and repressor of a pair of coregulated exons: promotes inclusion of the smooth muscle (SM) exon but exclusion of the non-muscle (NM) exon in actinin pre- mRNAs. Activates SM exon 5 inclusion by antagonizing the repressive effect of PTB. Promotes exclusion of exon 11 of the INSR pre-mRNA. Inhibits, together with HNRNPH1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Increases translation and controls the choice of translation initiation codon of CEBPB mRNA. Increases mRNA translation of CEBPB in aging liver. Increases translation of CDKN1A mRNA by antagonizing the repressive effect of CALR3. Mediates rapid cytoplasmic mRNA deadenylation. Recruits the deadenylase PARN to the poly(A) tail of EDEN-containing mRNAs to promote their deadenylation. Required for completion of spermatogenesis. Binds to (CUG)n triplet repeats in the 3'-UTR of transcripts such as DMPK and to Bruno response elements (BREs). Binds to muscle-specific splicing enhancer (MSE) intronic sites flanking the alternative exon 5 of TNNT2 pre-mRNA. Binds to AU-rich sequences (AREs or EDEN-like) localized in the 3'-UTR of JUN and FOS mRNAs. Binds to the IR RNA. Binds to the 5'-region of CDKN1A and CEBPB mRNAs. Binds with the 5'-region of CEBPB mRNA in aging liver. Belongs to the CELF/BRUNOL family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; RNA-binding; RNA processing

Chromosomal Location of Human Ortholog: 11p11

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleus; ribonucleoprotein complex

Molecular Function: mRNA binding; protein binding; translation initiation factor binding; RNA binding; translation repressor activity, nucleic acid binding; BRE binding; nucleotide binding

Biological Process: mRNA splice site selection; embryonic development; regulation of RNA splicing; negative regulation of translation; positive regulation of multicellular organism growth; mRNA processing; spermatid development; germ cell development; RNA interference