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Immunohistochemistry (IHC) (Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Mouse anti-Human CEACAM5 / CD66e Monoclonal Antibody | anti-CEACAM5 antibody

Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e

Gene Names
CEACAM5; CEA; CD66e
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Synonyms
CEACAM5 / CD66e; Monoclonal Antibody; Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e; Anti-CEACAM5 / CD66e Antibody (clone CB30) IHC-plus; CEACAM5; CD66e; CD66e antigen; CEA; Carcinoembryonic antigen; Meconium antigen 100; Human CEACAM5; anti-CEACAM5 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CB30
Specificity
Recognizes human CD66e, also known as carcinoembryonic antigen (CEA). CD66e is a member of the immunoglobulin supergene family that is mainly expressed on epithelial cells. CD66e may play a role in cancer cells metastasis, and has been used clinically as a tumor marker for early detection of disease
Purity/Purification
Affinity Purified
Form/Format
PBS, 0.09% sodium azide.
Concentration
1 mg/ml (varies by lot)
Sequence Length
702
Applicable Applications for anti-CEACAM5 antibody
Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Flo, IHC-Fr, IHC-P (10 ug/ml), IP
Usage: Immunohistology: This product requires protein digestion pre-treatment of paraffin sections e. g. pepsin.
Target Species
Human
Immunogen Description
Human carcinoembryonic antigen.
Immunogen Type
Purified protein
Immunogen
CEACAM5 / CD66e antibody was raised against human carcinoembryonic antigen.
Preparation and Storage
Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC) (Anti-CEA antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Product Categories/Family for anti-CEACAM5 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
76,724 Da
NCBI Official Full Name
carcinoembryonic antigen-related cell adhesion molecule 5 preproprotein
NCBI Official Synonym Full Names
carcinoembryonic antigen-related cell adhesion molecule 5
NCBI Official Symbol
CEACAM5
NCBI Official Synonym Symbols
CEA; CD66e
NCBI Protein Information
carcinoembryonic antigen-related cell adhesion molecule 5; meconium antigen 100
UniProt Protein Name
Carcinoembryonic antigen-related cell adhesion molecule 5
UniProt Gene Name
CEACAM5
UniProt Synonym Gene Names
CEA; CEA
UniProt Entry Name
CEAM5_HUMAN

NCBI Description

Members of the CEACAM subfamily, including CEACAM5, belong to the CEA gene family. For general information on the CEA gene family, see CEACAM1 (MIM 109770).[supplied by OMIM, Oct 2009]

Uniprot Description

CEACAM5: Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins. Homodimer. Binding of E.coli Dr adhesins leads to dissociation of the homodimer. Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon. Belongs to the immunoglobulin superfamily. CEA family.

Protein type: Immunoglobulin superfamily; Membrane protein, GPI anchor; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1-q13.2

Cellular Component: basolateral plasma membrane; integral to plasma membrane

Molecular Function: identical protein binding; protein homodimerization activity

Biological Process: homotypic cell-cell adhesion; negative regulation of apoptosis

Research Articles on CEACAM5

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Product Notes

The CEACAM5 ceacam5 (Catalog #AAA245861) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone CB30] (IgG1) to Human CEACAM5 / CD66e reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CEACAM5 / CD66e can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin; IHC - Frozen), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Flo, IHC-Fr, IHC-P (10 ug/ml), IP Usage: Immunohistology: This product requires protein digestion pre-treatment of paraffin sections e. g. pepsin. Researchers should empirically determine the suitability of the CEACAM5 ceacam5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CEACAM5 / CD66e, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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