Mouse CDK5 Monoclonal Antibody | anti-CDK5 antibody

CDK5(N-term) Monoclonal Antibody

Cat. Num. AAA9400180

• Gene Names: CDK5; LIS7; PSSALRE
• Reactivity: Human, Mouse, Rat, Porcine, Dog
• Applications: Western Blot, Immunocytochemistry
• Purity: Purified by antigen-affinity chromatography.
• Synonyms: CDK5; Monoclonal Antibody; CDK5(N-term) Monoclonal Antibody; cyclin-dependent kinase 5 TPKII catalytic subunit protein kinase CDK5 splicing cell division protein kinase 5 serine/threonine-protein kinase PSSALRE tau protein kinase II catalytic subunit; anti-CDK5 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat, Porcine, Dog
• Clonality: Monoclonal
• Isotype: IgG1
• Purity/Purification: Purified by antigen-affinity chromatography.
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 260

• Applicable Applications for anti-CDK5 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Western blotting: 1:1000
• Immunogen Type: Recombinant protein
• Immunogen Description: Recombinant fragment, corresponding to amino acids 1-138aaof human CDK5(N-terminus).
• Target Name: CDK5
• Preparation and Storage: store at -20 degree C

Testing Data

(1:1000 dilution of this antibody detected CDK5 on 40ug of cell lysates)

Testing Data

(HeLa cells using anti- CDK5 (N-terminus) antibody diluted 1:150)

Product Categories/Family for anti-CDK5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 256542306
• NCBI GeneID: 1020
• NCBI Accession #: NP_001157882.1
• NCBI GenBank Nucleotide #: NM_001164410.2
• UniProt Accession #: Q00535; A1XKG3
• Molecular Weight: 29,544 Da
• NCBI Official Full Name: cyclin-dependent-like kinase 5 isoform 2
• NCBI Official Synonym Full Names: cyclin-dependent kinase 5
• NCBI Official Symbol: CDK5
• NCBI Official Synonym Symbols: LIS7; PSSALRE
• NCBI Protein Information: cyclin-dependent-like kinase 5
• UniProt Protein Name: Cyclin-dependent-like kinase 5
• Protein Family: CDK5RAP1-like protein
• UniProt Gene Name: CDK5
• UniProt Synonym Gene Names: CDKN5; TPKII catalytic subunit
• UniProt Entry Name: CDK5_HUMAN

NCBI Description

This gene encodes a proline-directed serine/threonine kinase that is a member of the cyclin-dependent kinase family of proteins. Unlike other members of the family, the protein encoded by this gene does not directly control cell cycle regulation. Instead the protein, which is predominantly expressed at high levels in mammalian postmitotic central nervous system neurons, functions in diverse processes such as synaptic plasticity and neuronal migration through phosphorylation of proteins required for cytoskeletal organization, endocytosis and exocytosis, and apoptosis. In humans, an allelic variant of the gene that results in undetectable levels of the protein has been associated with lethal autosomal recessive lissencephaly-7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2015]

Uniprot Description

CDK5: a protein kinase of the CDK family. Unlike other members of this family, it is not activated by cyclins but by p35 (CDK5R1) and p39. An important regulator of neuronal positioning during brain development. May also play a role in synaptogenesis and neurotransmission. Substrates include TAU, MAP2, NF-H and -M, Nudel, PDE6, beta-catenin, amphyphysin, dynamin I, synapsin 1, Munc-18, and NMDA receptor 2A. Plays a role in myogenesis, haematopoietic cell differentiation, spermatogenesis, insulin secretion, and lens differentiation. Implicated in the pathology of neurofibrillary tangles and formation of senile plaques, hallmarks of Alzheimer?s disease. Induces tau phosphorylation and aggregation and neurofibrillary tangle deposition and neurodegeneration in in vitro and in vivo animal models. Brain samples from Alzeimer?s pateints show elevated CDK5 activity.

Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, CMGC; CMGC group; CDK family; CDK/CDK5 subfamily; CDK5 subfamily

Chromosomal Location of Human Ortholog: 7q36

Cellular Component: cyclin-dependent protein kinase 5 activator complex; postsynaptic density; dendrite; perikaryon; cytosol; postsynaptic membrane; cytoskeleton; growth cone; cell soma; membrane; axon; lamellipodium; cytoplasm; neuromuscular junction; nucleus; cell junction; filopodium

Molecular Function: acetylcholine receptor activator activity; ephrin receptor binding; p53 binding; protein kinase activity; protein serine/threonine kinase activity; ErbB-2 class receptor binding; protein binding; ErbB-3 class receptor binding; cyclin-dependent protein kinase activity; tau-protein kinase activity; cytoskeletal protein binding; kinase activity; ATP binding

Biological Process: Schwann cell development; negative regulation of synaptic plasticity; axon extension; cerebellar cortex formation; rhythmic process; regulation of synaptic plasticity; motor axon guidance; sensory perception of pain; regulation of cell migration; receptor clustering; regulation of apoptosis; neuron differentiation; corpus callosum development; receptor catabolic process; oligodendrocyte differentiation; neurite development; central nervous system neuron development; synaptic transmission, glutamatergic; skeletal muscle development; dendrite morphogenesis; cell division; synaptic vesicle endocytosis; negative regulation of protein ubiquitination; negative regulation of transcription, DNA-dependent; axon guidance; synaptic transmission, dopaminergic; regulated secretory pathway; negative regulation of protein export from nucleus; positive regulation of protein binding; cell-matrix adhesion; protein amino acid autophosphorylation; neuron migration; nucleocytoplasmic transport; negative regulation of axon extension; negative regulation of cell cycle; intracellular protein transport; synaptic transmission; synaptogenesis; behavioral response to cocaine; positive regulation of neuron apoptosis; visual learning; cortical actin cytoskeleton organization and biogenesis; layer formation in the cerebral cortex; serine phosphorylation of STAT3 protein; negative regulation of proteolysis; hippocampus development; peptidyl-threonine phosphorylation; cell cycle; cell proliferation; positive regulation of calcium ion-dependent exocytosis; embryonic development; peptidyl-serine phosphorylation; neuron apoptosis; synaptic vesicle exocytosis; positive regulation of protein kinase activity; regulation of excitatory postsynaptic membrane potential; blood coagulation; phosphorylation

Disease: Lissencephaly 7 With Cerebellar Hypoplasia