Mouse cdk4, p34 Monoclonal Antibody | anti-CDK2 antibody

cdk4, p34 (CDK4, Cyclin-dependent Kinase 4, p34cdk4)

Cat. Num. AAA602755

• Gene Names: CDK2; p33(CDK2)
• Reactivity: Human, Mouse, Porcine, Rat
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: cdk4; p34; Monoclonal Antibody; p34 (CDK4; Cyclin-dependent Kinase 4; p34cdk4); Anti -cdk4; anti-CDK2 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Porcine, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 0.N.199
• Specificity: Recognizes p34 (34kD). Does not crossreact with other cdks. Species Crossreactivity: Human, mouse, rat and porcine.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.1% sodium azide. Also available without BSA and azide. See C2574-05X.

• Applicable Applications for anti-CDK2 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunoprecipitation, Western Blot and Kinase assays.; Dilution: Immunoprecipitation Native only; coprecipitates cyclin D's: 2ug/mg protein lysate. Use protein G; Western Blot: 1-2ug/ml for 2 hours at RT.; Optiml dilutions to be determined by the researcher.
• Immunogen: Purified recombinant cdk4 protein. Cellular Localization: Nuclear and cyotplasmic. Epitope: aa1-20.
• Positive Control: MAD109, NIH3T3, LS174T, and MCF-7 cells.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot mouse cells)

Western Blot (WB)

(ip/Western Blot mousecells native)

Related Product Information for anti-CDK2 antibody

A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression.

Product Categories/Family for anti-CDK2 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 166362719
• NCBI GeneID: 1017
• NCBI Accession #: NP_439892.2
• NCBI GenBank Nucleotide #: NM_052827.2
• UniProt Accession #: P24941; O75100; A8K7C6
• Molecular Weight: 33,930 Da
• NCBI Official Full Name: cyclin-dependent kinase 2 isoform 2
• NCBI Official Synonym Full Names: cyclin-dependent kinase 2
• NCBI Official Symbol: CDK2
• NCBI Official Synonym Symbols: p33(CDK2)
• NCBI Protein Information: cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2
• UniProt Protein Name: Cyclin-dependent kinase 2
• Protein Family: Cyclin-dependent kinase
• UniProt Gene Name: CDK2
• UniProt Synonym Gene Names: CDKN2
• UniProt Entry Name: CDK2_HUMAN

NCBI Description

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

CDK2: a protein kinase of the CDK family. An important component of the cell cycle machinery. Activity of CDK2 is maximal during S phase and G2. Cdk2/cyclin E kinase activity is important for the G1 to S transition and phosphorylates the Rb protein. In S-phase, active cdk2/cyclin A complexes predominate and phosphorylate E2F, and the active cdk2 complex persists in the nucleus through G2. Part of the Rb pathway disregulated in most tumors. Target of several candidate cancer drugs. However, inhibition does not always prevent cancer cell growth, possibly due to CDK redundancy. Inhibitors: BMS-265246, BMS-265246-01, R-roscovitine (CYC200, CYC202), SU9516, R547, L868276

Protein type: Cell cycle regulation; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.22; Kinase, protein; CMGC group; CDK family; CDK1 subfamily; CDK/CDK1 subfamily

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: nucleoplasm; Y chromosome; Cajal body; centrosome; transcription factor complex; chromosome, telomeric region; X chromosome; cytoplasm; cyclin-dependent protein kinase holoenzyme complex; condensed chromosome; cytosol; nucleus; endosome

Molecular Function: cyclin binding; protein binding; cyclin-dependent protein kinase activity; metal ion binding; histone kinase activity; ATP binding

Biological Process: G1 DNA damage checkpoint; mitosis; meiosis; histone phosphorylation; positive regulation of transcription, DNA-dependent; DNA repair; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; peptidyl-serine phosphorylation; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of cell proliferation; Ras protein signal transduction; mitotic cell cycle; DNA replication; blood coagulation; G2/M transition of mitotic cell cycle; centrosome duplication; potassium ion transport; G1/S transition of mitotic cell cycle; positive regulation of DNA replication initiation