Mouse anti-Human CDC25C Monoclonal Antibody | anti-CDC25C antibody

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (AP)

Cat. Num. AAA6241554

• Gene Names: CDC25C; CDC25; PPP1R60
• Reactivity: Human
• Applications: Immunohistochemistry, Immunoprecipitation, Immunoblot, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CDC25C; Monoclonal Antibody; CDC25C (Cell Division Cycle 25 Homolog C; Cdc 25C; CDC 25; Dual Specificity Phosphatase Cdc25C; M-phase Inducer Phosphatase 3; MPIP3; PPP1R60) (AP); Cell Division Cycle 25C Protein; EC=3.1.3.48; Mitosis Inducer CDC25; anti-CDC25C antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 0.T.16
• Specificity: Recognizes human cdc25C doublet at 56kD and 59kD, as well as proteins at 40kD and 35kD. Species Crossreactivity: Human.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CDC25C antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunoblot
• Application Notes: IHC-P: IHC: Reported to immunostain cdc25C.; IP: Reported to immunoprecipitate cdc25C.; Applications are based on unconjugated antibody.; Immunoblot: 1-4ug/ml detects cdc25C in HeLa nuclear extract. HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed
• Immunogen: GST fusion-protein corresponding to residues 1-258 of human cdc25C. Epitope mapped to residues 222-246.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB: HeLa nuclear extract probed with MBS602875 at 1ug/ml.)

Product Categories/Family for anti-CDC25C antibody

Antibodies; Cell Division Cycle (CDC)

References

1. Dalal, S.N., et al., Mol. Cell Biol. 19: 4465-4479 (1999). 2. Peng, C.Y., et al., Science 277: 1501-1504 (1997). 3. O' Connor, P.M., et al., PNAS USA 91: 9480-9484 (1994). 4. Villa-Moruzzi, E., et al., Biochem. Biophys. Res. Commun. 196: 1248-1254 (1993).

NCBI and Uniprot Product Information

• NCBI GI #: 125625350
• NCBI GeneID: 995
• NCBI Accession #: NP_001781.2
• NCBI GenBank Nucleotide #: NM_001790
• UniProt Accession #: P30307; Q96PL3; Q9H168; Q9H2E8; Q9H2E9; Q9H2F1; D3DQB8
• Molecular Weight: 53,365 Da
• NCBI Official Full Name: M-phase inducer phosphatase 3 isoform a
• NCBI Official Synonym Full Names: cell division cycle 25C
• NCBI Official Symbol: CDC25C
• NCBI Official Synonym Symbols: CDC25; PPP1R60
• NCBI Protein Information: M-phase inducer phosphatase 3; mitosis inducer CDC25; phosphotyrosine phosphatase; dual specificity phosphatase CDC25C; protein phosphatase 1, regulatory subunit 60
• UniProt Protein Name: M-phase inducer phosphatase 3
• UniProt Gene Name: CDC25C
• UniProt Entry Name: MPIP3_HUMAN

NCBI Description

This gene is highly conserved during evolution and it plays a key role in the regulation of cell division. The encoded protein is a tyrosine phosphatase and belongs to the Cdc25 phosphatase family. It directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It is also thought to suppress p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described, however, the full-length nature of many of them is not known. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; EC 3.1.3.48; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 5q31

Cellular Component: nucleoplasm; perinuclear region of cytoplasm; nucleus; cytosol

Molecular Function: protein binding; WW domain binding; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: cell proliferation; mitosis; viral reproduction; regulation of cell cycle; cell division; regulation of mitosis; spermatogenesis; regulation of cyclin-dependent protein kinase activity; mitotic cell cycle; DNA replication; G2/M transition of mitotic cell cycle