Mouse anti-Human CDc20 (Cell Division Cycle Protein 20) Monoclonal Antibody | anti-CDC20 antibody

CDc20 (Cell Division Cycle Protein 20) Mouse Monoclonal Antibody

Cat. Num. AAA438179

• Gene Names: CDC20; CDC20A; p55CDC; bA276H19.3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
• Synonyms: CDc20 (Cell Division Cycle Protein 20); Monoclonal Antibody; CDc20 (Cell Division Cycle Protein 20) Mouse Monoclonal Antibody; CDC20; CDC20A; p55CDC; P55CDC-LSB; anti-CDC20 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: AR12
• Specificity: Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 499

• Applicable Applications for anti-CDC20 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cytoplasmic
• Immunogen: Urea-denatured His6 Cdc20 human recombinant protein
• Hu-Chromosome Location: 1p34.2
• Positive Control: Ramos or HeLa cells. Tonsil or gastric carcinoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with CDC20 Monoclonal Antibody (AR12))

References

Sironi L et al. 2001. EMBO J. 20(22):6371-82.

NCBI and Uniprot Product Information

• NCBI GI #: 118402582
• NCBI GeneID: 991
• NCBI Accession #: NP_001246.2
• NCBI GenBank Nucleotide #: NM_001255.2
• UniProt Accession #: Q12834; Q5JUY4; Q9BW56; Q9UQI9; B2R6Z6; D3DPJ1
• Molecular Weight: 55kDa
• NCBI Official Full Name: cell division cycle protein 20 homolog
• NCBI Official Synonym Full Names: cell division cycle 20
• NCBI Official Symbol: CDC20
• NCBI Official Synonym Symbols: CDC20A; p55CDC; bA276H19.3
• NCBI Protein Information: cell division cycle protein 20 homolog
• UniProt Protein Name: Cell division cycle protein 20 homolog
• Protein Family: Cell division cycle protein
• UniProt Gene Name: CDC20
• UniProt Entry Name: CDC20_HUMAN

NCBI Description

CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]

Uniprot Description

CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.

Protein type: Ubiquitin conjugating system; Cell cycle regulation

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm; nucleus; spindle

Molecular Function: enzyme binding; protein binding; protein C-terminus binding

Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion