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Flow Cytometry (FC/FACS) (Staining of BALB/c thymocytes with 0.5 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.5 ug of CD95 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)

Mouse anti-Mouse CD95 Monoclonal Antibody | anti-CD95 antibody

Mouse monoclonal CD95 antibody

Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
CD95; Monoclonal Antibody; Mouse monoclonal CD95 antibody; CD95 antibody; anti-CD95 antibody
Ordering
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
15A7
Specificity
Mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
11
Applicable Applications for anti-CD95 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC: <= 0.5 ug/test
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10? to 10? cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.

Flow Cytometry (FC/FACS)

(Staining of BALB/c thymocytes with 0.5 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.5 ug of CD95 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)

Flow Cytometry (FC/FACS) (Staining of BALB/c thymocytes with 0.5 ug of Mouse IgG1 kappa Isotype Control (open histogram) or 0.5 ug of CD95 antibody (filled histogram) followed by Anti-Mouse IgG (FITC). Total viable cells were used for analysis.)
Product Categories/Family for anti-CD95 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
355
UniProt Accession #
Molecular Weight
1,256 Da
NCBI Official Full Name
CD95 antigen, partial
NCBI Official Synonym Full Names
Fas cell surface death receptor
NCBI Official Symbol
FAS
NCBI Official Synonym Symbols
APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
NCBI Protein Information
tumor necrosis factor receptor superfamily member 6
UniProt Protein Name
CD95 antigen
UniProt Gene Name
CD95

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. Several alternatively spliced transcript variants have been described, some of which are candidates for nonsense-mediated mRNA decay (NMD). The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform. [provided by RefSeq, Mar 2011]

Research Articles on CD95

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Product Notes

The CD95 cd95 (Catalog #AAA5305661) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal CD95 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD95 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB). FC: <= 0.5 ug/test. Researchers should empirically determine the suitability of the CD95 cd95 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD95, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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