Rabbit anti-Rat CD93 Monoclonal Antibody | anti-CD93 antibody

Recombinant Anti-CD93 Antibody (FITC), Rabbit Monoclonal

Cat. Num. AAA8112824

• Gene Names: Cd93; Ly68; C1qRp; C1qr1
• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD93; Monoclonal Antibody; Recombinant Anti-CD93 Antibody (FITC); Rabbit Monoclonal; CD93/C1qR Antibody (FITC); Rabbit MAb; CD93 molecule; anti-CD93 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 138
• Specificity: Rat CD93/C1qR
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 5ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD93 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Rat CD93/C1qR Protein
• Conjugation: FITC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Rat CD93/C1qR (rR CD93/C1qR) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Rat CD93 expression on SD rat bone marrow cells. Cells were stained with FITC-conjugated anti-Rat CD93. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-CD93 antibody

CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.

References

Bohlson SS, et al. (2005) CD93 is rapidly shed from the surface of human myeloid cells and the soluble form is detected in human plasma. J Immunol. 175(2): 1239-47.Norsworthy PJ, et al. (2004) Murine CD93 (C1qRp) contributes to the removal of apoptotic cells in vivo but is not required for C1q-mediated enhancement of phagocytosis. J Immunol. 172(6): 3406-14.Chevrier S, et al. (2009) CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche. Proc Natl Acad Sci U S A. 106(10): 3895-900.

NCBI and Uniprot Product Information

• NCBI GI #: 16758114
• NCBI GeneID: 84398
• NCBI Accession #: NP_445835.1
• NCBI GenBank Nucleotide #: NM_053383.1
• UniProt Accession #: Q9ET61; Q9JIZ6
• Molecular Weight: 68,782 Da
• NCBI Official Full Name: complement component C1q receptor
• NCBI Official Synonym Full Names: CD93 molecule
• NCBI Official Symbol: Cd93
• NCBI Official Synonym Symbols: Ly68; C1qRp; C1qr1
• NCBI Protein Information: complement component C1q receptor
• UniProt Protein Name: Complement component C1q receptor
• Protein Family: Complement component C1q receptor
• UniProt Gene Name: Cd93
• UniProt Synonym Gene Names: C1qr1; C1qrp; C1qR(p); C1qRp
• UniProt Entry Name: C1QR1_RAT

NCBI Description

enhances cell-cell interactions; may play a role in innate immune response against pathogens and toxic cell debris [RGD, Feb 2006]

Uniprot Description

CD93: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell surface; cytoplasmic membrane-bound vesicle; integral to membrane; plasma membrane

Molecular Function: complement component C1q binding; calcium ion binding; carbohydrate binding

Biological Process: cell-cell adhesion