CD93 recombinant protein

CD93 Protein, Mouse, Recombinant (ECD, His Tag)

Cat. Num. AAA8118865

• Gene Names: Cd93; Ly68; AA4.1; C1qr1; C1qrp; AA145088; AW555904; 6030404G09Rik
• Purity: >90 % as determined by SDS-PAGE.
• Synonyms: CD93; CD93 Protein; Mouse; Recombinant (ECD; His Tag); Mouse CD93/C1qR Protein (ECD; CD93 molecule; 6030404G09Rik Protein; AA145088 Protein; AA4.1 Protein; AW555904 Protein; C1qr1 Protein; C1qrp Protein; Ly68 Protein; CD93 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Sequence Positions: 561aa
• Sequence: Met1-Asn572

• Species: Mouse
• Tag: C-His
• Endotoxin: <1.0EU per ug protein as determined by the LAL method
• Predicted N Terminal: Ala23
• Protein Construction: |A DNA sequence encoding the mouse CD93 (NP_034870.1) (Met1-Asn572) was expressed with a polyhistidine tag at the C-terminus.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; Shipping: They are shipped out with blue ice pack

Testing Data

Related Product Information for CD93 recombinant protein

CD93 or C1q receptor 1 (C1qR) is an about 120 kDa O-sialoglycoprotein that within the hematopoietic system is selectively expressed on cells of the myeloid lineage. CD93/C1qR is a highly glycosylated transmembrane protein expressed on monocytes, neutrophils, endothelial cells, and stem cells. CD93 was originally identified as a myeloid cell-surface marker and subsequently associated with an ability to modulate phagocytosis of suboptimally opsonized immunoglobulin G and complement particles in vitro. CD93/C1qR, a receptor expressed during early B-cell development, is reinduced during plasma-cell differentiation. High CD93/CD138 expression was restricted to antibody-secreting cells both in T-dependent and T-independent responses as naive, memory, and germinal-center B cells remained CD93-negative. CD93 was expressed on (pre)plasmablasts/plasma cells, including long-lived plasma cells that showed decreased cell cycle activity, high levels of isotype-switched Ig secretion, and modification of the transcriptional network. CD93 is important for the maintenance of plasma cells in bone marrow niches.

References

Bohlson SS, et al. (2005) CD93 is rapidly shed from the surface of human myeloid cells and the soluble form is detected in human plasma. J Immunol. 175(2): 1239-47.Norsworthy PJ, et al. (2004) Murine CD93 (C1qRp) contributes to the removal of apoptotic cells in vivo but is not required for C1q-mediated enhancement of phagocytosis. J Immunol. 172(6): 3406-14.Chevrier S, et al. (2009) CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche. Proc Natl Acad Sci U S A. 106(10): 3895-900.

NCBI and Uniprot Product Information

• NCBI GI #: 6754578
• NCBI GeneID: 17064
• NCBI Accession #: NP_034870.1
• NCBI GenBank Nucleotide #: NM_010740.3
• UniProt Accession #: O89103; Q3U2X0; A2AVY5
• Molecular Weight: 69,354 Da
• NCBI Official Full Name: complement component C1q receptor
• NCBI Official Synonym Full Names: CD93 antigen
• NCBI Official Symbol: Cd93
• NCBI Official Synonym Symbols: Ly68; AA4.1; C1qr1; C1qrp; AA145088; AW555904; 6030404G09Rik
• NCBI Protein Information: complement component C1q receptor
• UniProt Protein Name: Complement component C1q receptor
• Protein Family: Complement component C1q receptor
• UniProt Gene Name: Cd93
• UniProt Synonym Gene Names: Aa4; C1qr1; C1qrp; Ly68; C1qR(p); C1qRp; Ly-68
• UniProt Entry Name: C1QR1_MOUSE

Uniprot Description

CD93: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Protein type: Cell adhesion; Membrane protein, integral

Cellular Component: cell surface; cytoplasmic vesicle; extracellular matrix; plasma membrane

Molecular Function: complement component C1q binding

Biological Process: cell-cell adhesion