Mouse anti-Human, Cow CD90 / Thy1 Monoclonal Antibody | anti-THY1 antibody

CD90 / Thy1 (Stromal / Mesenchymal Marker) Mouse Monoclonal Antibody

Cat. Num. AAA439589

• Gene Names: THY1; CD90; CDw90
• Reactivity: Human, Cow
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD90 / Thy1; Monoclonal Antibody; CD90 / Thy1 (Stromal / Mesenchymal Marker) Mouse Monoclonal Antibody; CD90; Theta antigen; Thy 1 cell surface antigen; Thy 1 membrane glycoprotein; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy1; Thy1 T cell antigen; Thy1.1; Thy1.2; Thymus cell antigen 1; theta; anti-THY1 antibody

• Host: Mouse
• Reactivity: Human, Cow
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: AF-9
• Specificity: Recognizes a protein of 18-35kDa, identified as CD90 (also known as Thy1). CD90 is a member of the immunoglobulin superfamily. It may contribute to inhibition of proliferation/differentiation of hematopoietic stem cells and neuron memory formation in the CNS. It consists of a single Ig domain (112 amino acids; 25-35 kDa) inserted into the cell membrane via a GPI anchor. Expressed by hematopoietic stem cells and neurons in all species studied. Its highly expressed in connective tissue and various fibroblast and stromal cell lines, expressed on all thymocytes and peripheral T cells in mice, but in humans expressed only on small % fetal thymocytes, 10-40% of CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in peripheral circulation. It is also expressed by human lymph node HEV endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 161

• Applicable Applications for anti-THY1 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (1-2ug/million cells in 0.1ml); Immunofluorescence (1-2ug/ml)
• Cellular Localization: Cell Surface
• Immunogen: Human T-lymphoma cells
• Hu-Chromosome Location: 11q23.3
• Positive Control: IMR-32, CCRF-CEM or MOLT-4 cells. Human uterus.
• Preparation and Storage: Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-PAGE

(SDS-PAGE Analysis Purified CD90 Mouse Monoclonal antibody (AF-9). Confirmation of Purity and Integrity of Antibody.)

References

Beissert S et al. Impaired cutaneous immune responses in Thy-1-deficient mice. J Immunol 1998, 161(10):5296-302 Williams, A.F., and J. Gagnon. 1982. Neuronal cell Thy-1 glycoprotein: Homology with immunoglobulin. Science 216: 696 - 703 Fujita N et al. Aggregation of Thy-1 glycoprotein induces thymocyte apoptosis through activation of CPP32-like proteases. Exp Cell Res 1997, 232(2):400-406 Kroczek, R.A., K.C. Gunter, R.N. Germain, and E.M. Shevach. 1986. Thy-1 functions as a signal transduction molecule in T lymphocytes and transfected B lymphocytes. Nature 322: 181 - 184

NCBI and Uniprot Product Information

• NCBI GI #: 902967471
• NCBI GeneID: 7070
• NCBI Accession #: NP_001298089.1
• NCBI GenBank Nucleotide #: NM_001311160.1
• UniProt Accession #: P04216; Q16008; Q9NSP1
• Molecular Weight: 18-35kDa
• NCBI Official Full Name: thy-1 membrane glycoprotein preproprotein
• NCBI Official Synonym Full Names: Thy-1 cell surface antigen
• NCBI Official Symbol: THY1
• NCBI Official Synonym Symbols: CD90; CDw90
• NCBI Protein Information: thy-1 membrane glycoprotein
• UniProt Protein Name: Thy-1 membrane glycoprotein
• Protein Family: Thy-1 membrane glycoprotein
• UniProt Gene Name: THY1
• UniProt Entry Name: THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; apical plasma membrane; dendrite; endoplasmic reticulum; external side of plasma membrane; focal adhesion; growth cone; integral to plasma membrane; lipid raft; myelin sheath; plasma membrane

Molecular Function: GTPase activator activity; integrin binding; protein binding; protein kinase binding

Biological Process: angiogenesis; cell-cell adhesion; cytoskeleton organization and biogenesis; focal adhesion formation; negative regulation of axonogenesis; negative regulation of cell migration; negative regulation of protein kinase activity; negative regulation of T cell receptor signaling pathway; positive regulation of GTPase activity; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of T cell activation; retinal cone cell development; T cell receptor signaling pathway