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Mouse Cd90 Monoclonal Antibody | anti-Cd90 antibody

Cd90 (T25, Theta antigen, Thy-1 antigen,Thy-1 membrane glycoprotein, Thy1 T cell antigen, Thy1.1, Thy1.2) (AP)

Gene Names
THY1; CD90
Reactivity
Human, Monkey
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Purity
Purified by Protein A/G Affinity Chromatography.
Synonyms
Cd90; Monoclonal Antibody; Cd90 (T25; Theta antigen; Thy-1 antigen; Thy-1 membrane glycoprotein; Thy1 T cell antigen; Thy1.1; Thy1.2) (AP); T25; Thy1.2; anti-Cd90 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
F15-42-1
Specificity
Recognizes human Cd90. Species Crossreactivity: monkey. Others not known.
Purity/Purification
Purified by Protein A/G Affinity Chromatography.
Form/Format
Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-Cd90 antibody
Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP)
Application Notes
IP: 0.5-1ug/500ug protein lysate
IF: 0.5-1ug/ml
IHC-F: 0.5-1.0ug/ml for 30 minutes at RT
Applications are based on unconjugated antibody.
Immunogen
Purified human brain Thy1. Cellular Localization: Cell surface
Conjugate
AP
Positive Control
MOLT4 or HUT78 cells. Human cerebellum.
Preparation and Storage
May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Freezing alkaline phosphatase conjugates will result in a substantial loss of activity.
Product Categories/Family for anti-Cd90 antibody
References
1. Cervello I et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One6:e21221 (2011). 2. Lehmann GM et al. Novel anti-adipogenic activity produced by human fibroblasts. Am J Physiol Cell Physiol 299:C672-81 (2010).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17,935 Da
NCBI Official Full Name
thy-1 membrane glycoprotein preproprotein
NCBI Official Synonym Full Names
Thy-1 cell surface antigen
NCBI Official Symbol
THY1
NCBI Official Synonym Symbols
CD90
NCBI Protein Information
thy-1 membrane glycoprotein; CDw90; thy-1 antigen; Thy-1 T-cell antigen
UniProt Protein Name
Thy-1 membrane glycoprotein
UniProt Gene Name
THY1
UniProt Entry Name
THY1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]

Uniprot Description

THY1: May play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain.

Protein type: Motility/polarity/chemotaxis; Membrane protein, GPI anchor; Cell adhesion

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to external side of plasma membrane; focal adhesion; growth cone; endoplasmic reticulum; integral to plasma membrane; dendrite; apical plasma membrane; plasma membrane; cytosol; lipid raft; external side of plasma membrane

Molecular Function: integrin binding; protein binding; protein kinase binding

Biological Process: focal adhesion formation; cell-cell adhesion; retinal cone cell development; negative regulation of T cell receptor signaling pathway; negative regulation of protein kinase activity; negative regulation of axonogenesis; cytoskeleton organization and biogenesis; angiogenesis; positive regulation of T cell activation; positive regulation of release of sequestered calcium ion into cytosol; T cell receptor signaling pathway; negative regulation of cell migration; positive regulation of GTPase activity

Research Articles on Cd90

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Product Notes

The Cd90 thy1 (Catalog #AAA6122927) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Cd90 (T25, Theta antigen, Thy-1 antigen,Thy-1 membrane glycoprotein, Thy1 T cell antigen, Thy1.1, Thy1.2) (AP) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Cd90 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC) Frozen, Immunoprecipitation (IP). IP: 0.5-1ug/500ug protein lysate IF: 0.5-1ug/ml IHC-F: 0.5-1.0ug/ml for 30 minutes at RT Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Cd90 thy1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cd90, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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