Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) Alexa Fluor 488 antibody (concentration in sample 10 ug/ml).)
Mouse anti-Human, Cow CD9 Monoclonal Antibody | anti-CD9 antibody
Anti-Bov CD9 Alexa Fluor488
Reactivity
Human, Cow
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD9; Monoclonal Antibody; Anti-Bov CD9 Alexa Fluor488; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; CD9 molecule; anti-CD9 antibody
Host
Mouse
Reactivity
Human, Cow
Clonality
Monoclonal
Isotype
Mouse IgG2
Clone Number
IVA50
Specificity
The antibody IVA50 reacts with an extracellular epitope of CD9 antigen (bovine), a 24kDa single transmembrane polypeptide expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes. This antibody crossreacts with CD9 on human platelets.
Purity/Purification
The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Phosphate buffered saline (PBS) solution with 15mM sodium azide
Concentration
0.5mg/ml (varies by lot)
Applicable Applications for anti-CD9 antibody
Flow Cytometry (FC/FACS) (QC tested)
Conjugation
Alexa Fluor488
Immunogen
Bovine thrombocytes.
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) Alexa Fluor 488 antibody (concentration in sample 10 ug/ml).)
Flow Cytometry (FC/FACS)
(Flow cytometry surface staining pattern of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) Alexa Fluor 488 antibody (concentration in sample 10 ug/ml).)
Flow Cytometry (FC/FACS)
(Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) Alexa Fluor 488 antibody (concentration in sample 10 ug/ml).)
Flow Cytometry (FC/FACS)
(Separation of bovine CD9 positive thrombocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of bovine peripheral whole blood stained using anti-bovine CD9 (IVA50) Alexa Fluor 488 antibody (concentration in sample 10 ug/ml).)
Related Product Information for anti-CD9 antibody
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
References
*Sopp P, Howard CJ: Investigation of PC36 (BoCD45R). Vet Immunol Immunopathol. 1996 Aug;52(4):271-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896215dopt=A,*Naessens J & Hopkins J: Proceedings of the 3rd workshop on ruminant leukocyte antigens. Davis, California, 16 July 1995. Vet Immunol Immunopathol 52:213-468 (1996), URL: https://www.ncbi.nlm.nih.gov/pubmed/8896204dopt=A,*Naessens J, Howard CJ, Hopkins J: Nomenclature and characterization of leukocyte differentiation antigens in ruminants. Immunol Today. 1997 Aug;18(8):365-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9267076dopt=A,*Tomaskova J, Dusinsky R, Horovska L, Simon M: A set of monoclonal antibodies specific for bovine cell surface molecule CD9. Folia Biol (Praha). 1999;45(5):225-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/10730891dopt=,*Mateo A, Perez de la Lastra J, Moreno A, Dusinsky R, Bilka F, Simon M, Horovska L, Llanes D: Biochemical characterization of antigens detected with anti-platelet monoclonal antibodies. Vet Immunol Immunopathol. 1996 Aug;52(4):363-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/8896226dopt=A
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25,258 Da
NCBI Official Full Name
CD9 antigen
NCBI Official Synonym Full Names
CD9 molecule<
NCBI Official Symbol
CD9
NCBI Protein Information
CD9 antigen; CD9 antigen (p24)
UniProt Protein Name
CD9 antigen
Protein Family
UniProt Gene Name
CD9
UniProt Entry Name
CD9_BOVIN
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Product Notes
The CD9 cd9 (Catalog #AAA1801970) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Bov CD9 Alexa Fluor488 reacts with Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's CD9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS) (QC tested). Researchers should empirically determine the suitability of the CD9 cd9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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