Mouse anti-Human CD8a Monoclonal Antibody | anti-CD8a antibody

Anti-Human CD8a Monoclonal Antibody (FITC Conjugated)

Cat. Num. AAA2553959

• Gene Names: CD8A; CD8; p32; Leu2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD8a; Monoclonal Antibody; Anti-Human CD8a Monoclonal Antibody (FITC Conjugated); Human CD8a; T-cell surface glycoprotein CD8 alpha chain; CD8A; T-lymphocyte differentiation antigen T8/Leu-2; MAL; anti-CD8a antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: OKT-8
• Form/Format: Liquid; PBS with 0.05% Proclin300, 1% BSA
• Sequence Length: 235

• Applicable Applications for anti-CD8a antibody: Flow Cytometry (FC/FACS)
• Application Notes: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 muL of antibody per test (million cells in 100 ul staining volume or per 100 ul of whole blood). Please check your vial before the experiment.
• Conjugation: FITC
• Cellular Localization: Membrane
• Preparation and Storage: Store at 2-8 degree C. Protected from prolonged exposure to light.

Related Product Information for anti-CD8a antibody

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the alpha3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

References

1. Knapp W. W. Knapp. et al.Oxford University Press; 1989; :1-1182. 2. Reimann KA, Waite BC, Lee-Parritz DE, et al. Cytometry. 1994; 17(1):102-108. 3. Roederer M, Kantor AB, Parks DR, Herzenberg LA. Cytometry. 1996; 24(3):191-197. 4. Schlossman SF. Stuart F. Schlossman. et al. Oxford University Press; 1995. 5. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Cytometry. 1997; 29(4):351-362.

NCBI and Uniprot Product Information

• NCBI GI #: 225007536
• NCBI GeneID: 925
• NCBI Accession #: NP_001139345.1
• NCBI GenBank Nucleotide #: NM_001145873.1
• UniProt Accession #: P01732; Q13970; Q4ZG17; B4DT80; D6W5M8
• Molecular Weight: 21,585 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD8 alpha chain isoform 1
• NCBI Official Synonym Full Names: CD8a molecule
• NCBI Official Symbol: CD8A
• NCBI Official Synonym Symbols: CD8; p32; Leu2
• NCBI Protein Information: T-cell surface glycoprotein CD8 alpha chain
• UniProt Protein Name: T-cell surface glycoprotein CD8 alpha chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD8A
• UniProt Synonym Gene Names: MAL

NCBI Description

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

Uniprot Description

Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). This mechanism enables CTLs to recognize and eliminate infected cells and tumor cells. In NK-cells, the presence of CD8A homodimers at the cell surface provides a survival mechanism allowing conjugation and lysis of multiple target cells. CD8A homodimer molecules also promote the survival and differentiation of activated lymphocytes into memory CD8 T-cells.