Mouse CD88 Monoclonal Antibody | anti-CD88 antibody

CD88 (CD88 Antigen, Anaphylatoxin Receptor, C5a Anaphylatoxin Chemotactic Receptor, C5aR, C5a-R, C5a Ligand, C5R1) (Biotin)

Cat. Num. AAA6248794

• Gene Names: C5AR1; C5A; C5AR; C5R1; CD88
• Reactivity: Human, Monkey
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: CD88; Monoclonal Antibody; CD88 (CD88 Antigen; Anaphylatoxin Receptor; C5a Anaphylatoxin Chemotactic Receptor; C5aR; C5a-R; C5a Ligand; C5R1) (Biotin); Complement Component 5 Receptor 1; Complement Component 5 Receptor 2; anti-CD88 antibody

• Host: Mouse
• Reactivity: Human, Monkey
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3H1740
• Specificity: Recognizes human the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Does not inhibit the binding of C5a to its receptor. Species Crossreactivity: Rhesus Monkey
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

• Applicable Applications for anti-CD88 antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Immunogen: C5aR-peptide: Met1-Asn31
• Conjugate: Biotin
• Preparation and Storage: Store at -20 degree C.

Testing Data

(Staining of human peripheral blood granulocytes with Human CD88: Alexa Fluor® 647)

Testing Data

(Staining of human peripheral blood granulocytes with C2439-61E.)

Testing Data

(Staining of human peripheral blood granulocytes with Mouse anti-human (FITC) CD88)

Product Categories/Family for anti-CD88 antibody

Antibodies; CD Markers

References

1. Opperman, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain J. Immunol. 151: 3785 - 3794, 2. Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity. In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P: 955-956, 3. Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin. In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P.: 957-959, 4. Oppermann, M. (1995) Cluster report: (C5a receptor). In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. 953-954, 5. Oppermann, M. and Gotze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors Immunology. 82: 516-521, 6. Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin. J Immunol. 157: 1729-35.

NCBI and Uniprot Product Information

• NCBI GI #: 4502509
• NCBI GeneID: 728
• NCBI Accession #: NP_001727.1
• NCBI GenBank Nucleotide #: NM_001736.3
• UniProt Accession #: P21730
• Molecular Weight: 39,336 Da
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor
• NCBI Official Synonym Full Names: complement component 5a receptor 1
• NCBI Official Symbol: C5AR1
• NCBI Official Synonym Symbols: C5A; C5AR; C5R1; CD88
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR1
• UniProt Synonym Gene Names: C5AR; C5R1; C5a-R; C5aR
• UniProt Entry Name: C5AR_HUMAN

Uniprot Description

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; activation of MAPK activity; apoptosis; response to lipopolysaccharide; chemotaxis; cell proliferation in hindbrain; signal transduction; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation