Testing Data
(Fig.1: FACS analysis of Dubca cells using QV-6A8-S3 Cat. # GM-1113. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve).)
Rat anti-Human CD81 Monoclonal Antibody | anti-CD81 antibody
anti-human CD81 monoclonal antibody
Flow Cytometry: 1.2 ug/10^6 cells
CELISA: 1:200 - 1:400
Testing Data
(Fig.1: FACS analysis of Dubca cells using QV-6A8-S3 Cat. # GM-1113. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve).)
Testing Data
(Fig.1: FACS analysis of Dubca cells using QV-6A8-S3 Cat. # GM-1113. Dubca cells were transiently transfected with an expression vector encoding CD81 (red curve). Binding of QV-6A8-S3 was detected with a PE-conjugated secondary antibody. (Isotype control: black curve).)
Testing Data #2
(Fig.3: SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Testing Data #2
(Fig.3: SDS-PAGE analysis of purified QV-6A8-S3 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ?g of purified QV-6A8-S3 antibody. Proteins were separated by SDSPAGE and stained with RAPID StainTM Reagent.)
Pileri P, Uematsu Y, Campagnoli S, Galli G, Falugi F, Petracca R, Weiner AJ, Houghton M, Rosa D, Grandi G and Abrignana S (1998). Binding of Hepatitis C Virus to CD81. Science 282 no. 5390 pp. 938-941
Flint M, von Hahn T, Zhang J, Farquhar M, Jones CT, Balfe P, Rice CM and McKeating JA (2006). Diverse CD81 Proteins Support Hepatitis C Virus Infection. Journal of Virology p.11331- 11342
Fofana I, Xiao F, Thumann C, Turek M, Zona L, Tawar RG, Grunert F, Thompson J, Zeisel MB, Baumert TF (2013). A Novel Monoclonal Anti-CD81 Antibody Produced by Genetic Immunization Efficiently Inhibits Hepatitis C Virus Cell-Cell Transmission. PLoS One 21;8(5):e64221
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]
Uniprot Description
CD81: May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May act as the viral receptor for HCV. Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low. Belongs to the tetraspanin (TM4SF) family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; immunological synapse; vesicle
Molecular Function: protein binding
Biological Process: virion attachment, binding of host cell surface receptor; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; protein localization; regulation of immune response; entry of virus into host cell; activation of MAPK activity; positive regulation of cell proliferation; positive regulation of 1-phosphatidylinositol 4-kinase activity; phosphatidylinositol biosynthetic process; positive regulation of B cell proliferation; phosphatidylinositol metabolic process
Disease: Immunodeficiency, Common Variable, 6
Research Articles on CD81
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Product Notes
The CD81 cd81 (Catalog #AAA468029) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD81 monoclonal antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD81 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), CELISA. ELISA: 1:200 - 1:400 Flow Cytometry: 1.2 ug/10^6 cells CELISA: 1:200 - 1:400. Researchers should empirically determine the suitability of the CD81 cd81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD81, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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