anti-Mouse CD79b Monoclonal Antibody | anti-CD79b antibody

Anti-Ms CD79b Purified

• Gene Names: Cd79b; B29; Igb; Igbeta; Ig-beta
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunocytochemistry
• Purity: Purified by protein-A affinity chromatography
• Synonyms: CD79b; Monoclonal Antibody; Anti-Ms CD79b Purified; BCR beta; Ig-beta; B29; IGB; CD79b molecule; anti-CD79b antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG2
• Clone Number: HM79
• Specificity: The Armenian hamster monoclonal antibody HM79 recognizes an extracellular epitope of mouse CD79b (CD79 beta, Ig beta), a component of B cell receptor (BCR) complex.
• Purity/Purification: Purified by protein-A affinity chromatography
• Form/Format: Purified. Phosphate buffered saline (PBS) solution with 15mM sodium azide
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD79b antibody: Flow Cytometry (FC/FACS) (QC tested), Immunoprecipitation (IP), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: FC/FACS: Recommended dilution: 1ug/ml
• Negative Species: Human
• Immunogen: Purified CD79a/b (alpha/beta) dimers from WEHI231 cells
• Preparation and Storage: Store at 2-8 degree C. Do not freeze. Do not use after expiration date stamped on the label.

Related Product Information for anti-CD79b antibody

CD79b (Ig beta, B29) forms disulfide-linked heterodimer with CD79a (Ig alpha, MB1). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.

References

*Kouro T, Nagata K, Takaki S, Nisitani S, Hirano M, Wahl MI, Witte ON, Karasuyama H, Takatsu K: Bruton's tyrosine kinase is required for signaling the CD79b-mediated pro-B to pre-B cell transition. Int Immunol. 2001 Apr;13(4):485-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/11282988itool,*Koyama M, Ishihara K, Karasuyama H, Cordell JL, Iwamoto A, Nakamura T: CD79 alpha/CD79 beta heterodimers are expressed on pro-B cell surfaces without associated mu heavy chain. Int Immunol. 1997 Nov;9(11):1767-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/9418137ordina,*Maki K, Nagata K, Kitamura F, Takemori T, Karasuyama H: Immunoglobulin beta signaling regulates locus accessibility for ordered immunoglobulin gene rearrangements. J Exp Med. 2000 Apr 17;191(8):1333-40., URL: https://www.ncbi.nlm.nih.gov/pubmed/10770800ordin,*Li Y, Chen F, Putt M, Koo YK, Madaio M, Cambier JC, Cohen PL, Eisenberg RA: B cell depletion with anti-CD79 mAbs ameliorates autoimmune disease in MRL/lpr mice. J Immunol. 2008 Sep 1;181(5):2961-72., URL: https://www.ncbi.nlm.nih.gov/pubmed/18713966ordin,*Aiba Y, Kameyama M, Yamazaki T, Tedder TF, Kurosaki T: Regulation of B-cell development by BCAP and CD19 through their binding to phosphoinositide 3-kinase. Blood. 2008 Feb 1;111(3):1497-503., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025150itool
*Kouro T, Nagata K, Takaki S, Nisitani S, Hirano M, Wahl MI, Witte ON, Karasuyama H, Takatsu K: Bruton's tyrosine kinase is required for signaling the CD79b-mediated pro-B to pre-B cell transition. Int Immunol. 2001 Apr;13(4):485-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/11282988ordin,*Fuentes-Pananá EM, Bannish G, van der Voort D, King LB, Monroe JG: Ig alpha/Ig beta complexes generate signals for B cell development independent of selective plasma membrane compartmentalization. J Immunol. 2005 Feb 1;174(3):1245-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/15661879ordin,*Fuentes-Pananá EM, Bannish G, Shah N, Monroe JG: Basal Igalpha/Igbeta signals trigger the coordinated initiation of pre-B cell antigen receptor-dependent processes. J Immunol. 2004 Jul 15;173(2):1000-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/15240688ordin,*Nagata K, Nakamura T, Kitamura F, Kuramochi S, Taki S, Campbell KS, Karasuyama H: The Ig alpha/Igbeta heterodimer on mu-negative proB cells is competent for transducing signals to induce early B cell differentiation. Immunity. 1997 Oct;7(4):559-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/9354476ordina,*Teh YM, Neuberger MS: The immunoglobulin (Ig)alpha and Igbeta cytoplasmic domains are independently sufficient to signal B cell maturation and activation in transgenic mice. J Exp Med. 1997 May 19;185(10):1753-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/9151700ordina,*Gong S, Nussenzweig MC: Regulation of an early developmental checkpoint in the B cell pathway by Ig beta. Science. 1996 Apr 19;272(5260):411-4., URL: https://www.ncbi.nlm.nih.gov/pubmed/8602530ordina

NCBI and Uniprot Product Information

• NCBI GI #: 6680375
• NCBI GeneID: 15985
• NCBI Accession #: NP_032365.1
• NCBI GenBank Nucleotide #: NM_008339.2
• UniProt Accession #: P15530; Q4FJP4
• Molecular Weight: 25,726 Da
• NCBI Official Full Name: B-cell antigen receptor complex-associated protein beta chain
• NCBI Official Synonym Full Names: CD79B antigen
• NCBI Official Symbol: Cd79b
• NCBI Official Synonym Symbols: B29; Igb; Igbeta; Ig-beta
• NCBI Protein Information: B-cell antigen receptor complex-associated protein beta chain; immunoglobulin-associated beta; B-cell-specific glycoprotein B29; immunoglobulin-associated B29 protein
• UniProt Protein Name: B-cell antigen receptor complex-associated protein beta chain
• Protein Family: B-cell antigen receptor complex-associated protein
• UniProt Gene Name: Cd79b
• UniProt Synonym Gene Names: Igb
• UniProt Entry Name: CD79B_MOUSE