Mouse anti-Human CD74 Monoclonal Antibody | anti-CD74 antibody

CD74 Antibody, Clone 6D9: Alkaline Phosphatase

Cat. Num. AAA808493

• Gene Names: CD74; II; DHLAG; HLADG; Ia-GAMMA
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Protein G Purified
• Synonyms: CD74; Monoclonal Antibody; CD74 Antibody; Clone 6D9: Alkaline Phosphatase; Mouse Anti-Human CD74 Monoclonal IgG1; CD74 Antibody: Alkaline Phosphatase; DHLAG Antibody; HLA DR gamma Antibody; HLADG Antibody; p33 Antibody; p35 Antibody; Protein 41 Antibody; CD 74 Antibody; CD74 antigen (invariant polypeptide of major histocompatibility complex; class II antigen-associated) Antibody; CD74 antigen Antibody; CD74 molecule Antibody; CD74 molecule; major histocompatibility complex; class II invariant chain Antibody; CLIP Antibody; Gamma chain of class II antigens Antibody; HG2A_HUMAN Antibody; HLA class II histocompatibility antigen gamma chain Antibody; HLA DR antigens associated invariant chain Antibody; HLA-DR antigens-associated invariant chain Antibody; HLA-DR-gamma Antibody; HLADR antigens associated invariant chain Antibody; Ia antigen associated invariant chain Antibody; Ia antigen-associated invariant chain Antibody; Ia associated invariant chain Antibody; Ia gamma Antibody; Ii Antibody; Invariant polypeptide of major histocompatibility complex class II antigen associated Antibody; la-gamma Antibody; Major histocompatibility complex class II invariant chain Antibody; MHC HLA DR gamma chain Antibody; MHC HLA-DR gamma chain Antibody; anti-CD74 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D9
• Specificity: Detects ~33 kDa protein.
• Purity/Purification: Protein G Purified
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 160

• Applicable Applications for anti-CD74 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000; ICC/IF: 1:100
• Target: CD74
• Immunogen: Synthetic peptide corresponding to the N-terminal (1-100 aa) of human CD74 conjugated to KLH. Overlaps 100% with all 3 isoforms
• Immunogen Species: Human
• Cellular Localization: Cell Membrane; Endoplasmic Reticulum; Endoplasmic Reticulum Membrane; Golgi Apparatus; Endosome; Lysosome
• Conjugate: Alkaline Phosphatase
• Storage Buffer: PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
• Preparation and Storage: Store at -20 degree C.

Immunocytochemistry (ICC)

(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)

Western Blot (WB)

(Western Blot analysis of Human Lymphoblastoid cell line (Raji) showing detection of 33-35 kDa CD74 protein using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: Raji cell lysate. Load: 15 ug. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:1000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 33-35 kDa.)

Related Product Information for anti-CD74 antibody

CD74 is a non-polymorphic type II integral membrane protein. It has a short N-terminal cytoplasmic tail of 28 amino acids, followed by a single 24-aa transmembrane region and an approximately 150-aa lumenal domain (1). The CD74 chain is thought to function mainly as an MHC class II chaperone, which promotes ER exit of MHC class II molecules, directs them to endocytic compartments, prevents peptide binding in the ER, and contributes to peptide editing in the MHC class II compartment. Class II MHC and Ii expression was believed to be restricted to classical antigen-presenting cells (APC); however, during inflammation, other cell types, including mucosal epithelial cells, have also been reported to express class II MHC molecules (2). Experiments that investigate cell-surface CD74 are complicated by the fact that CD74 remains on the cell surface for a very short time. The surface half-life of CD74 was calculated to be fewer than 10 minutes (3). CD74 however has also recently been shown to have a role as an accessory-signaling molecule because of its high-affinity binding to the pro-inflammatory cytokine, macrophage migration-inhibitory factor (MIF) (3). The restricted expression of CD74 by normal tissues and its very rapid internalization make CD74 an attractive therapeutic target for both cancer and immunologic diseases (4).

Product Categories/Family for anti-CD74 antibody

Antibodies; Immunology; Adaptive Immunity; B Cells; B Cell CD Markers

References

1. Becker-Hermann, S., Arie, G., Medvedovsky H, Kerem A, and Shachar I. (2005) Mol Bio Cell. 16(11):5061-9. 2. Barrera CA., et al (2005) J Histochem Cytochem 53 (12): 1481-9. 3. Starlets D., et al. (2006) Blood. 107 (12): 4807-4816. 4. Burton JD., et al. (2004). Clin Cancer Res. 10(19): 6606-11. 5. Denzin L.K., Hammond, C. and Cresswell, P. (1996) J. Exp. Med. 184: 2153-2165. 6. Denzin L.K., Robbins N.F., Carboy-Newcomb C. and Cresswell P. (1994) Immunity 1: 595-606.

NCBI and Uniprot Product Information

• NCBI GI #: 68448537
• NCBI GeneID: 972
• NCBI Accession #: NP_001020329.1
• NCBI GenBank Nucleotide #: NM_001025158.2
• UniProt Accession #: P04233; Q14597; Q29832; Q5U0J8; Q8SNA0; Q8WLP6; A8K7R1; B4DNE8; D3DQG3; D3DQG4
• Molecular Weight: 18,328 Da
• NCBI Official Full Name: HLA class II histocompatibility antigen gamma chain isoform c
• NCBI Official Synonym Full Names: CD74 molecule
• NCBI Official Symbol: CD74
• NCBI Official Synonym Symbols: II; DHLAG; HLADG; Ia-GAMMA
• NCBI Protein Information: HLA class II histocompatibility antigen gamma chain
• UniProt Protein Name: HLA class II histocompatibility antigen gamma chain
• Protein Family: H-2 class II histocompatibility antigen
• UniProt Gene Name: CD74
• UniProt Synonym Gene Names: DHLAG; Ii

NCBI Description

The protein encoded by this gene associates with class II major histocompatibility complex (MHC) and is an important chaperone that regulates antigen presentation for immune response. It also serves as cell surface receptor for the cytokine macrophage migration inhibitory factor (MIF) which, when bound to the encoded protein, initiates survival pathways and cell proliferation. This protein also interacts with amyloid precursor protein (APP) and suppresses the production of amyloid beta (Abeta). Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

Plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to the endosomal/lysosomal system where the antigen processing and binding of antigenic peptides to MHC class II takes place. Serves as cell surface receptor for the cytokine MIF.