CHL-1 recombinant protein

Recombinant Human CHL-1 Protein (His tag)

Cat. Num. AAA2546488

• Gene Names: CHL1; CALL; L1CAM2
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: CHL-1; Recombinant Human CHL-1 Protein (His tag); CALL; FLJ44930; L1CAM2; MGC132578; CHL-1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1208

• Application Notes: The recombinant human CHL1 consists of 1067 amino acids after removal of the signal peptide and predicts a molecular mass of 120 kDa. As a result of glycosylation, the apparent molecular mass of rhCHL1 is approximately 160-180 kDa in SDS-PAGE under non-reduced conditions.
• Predicted N Terminal: Ile 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CHL-1 recombinant protein

Background: Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin type III domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.

Description: A DNA sequence encoding the extracellular domain of human CHL1 (AAI04919.1) (Met 1-Gln 1080) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 85397970
• NCBI GeneID: 10752
• Molecular Weight: 136,698 Da
• NCBI Official Full Name: CHL1 protein
• NCBI Official Synonym Full Names: cell adhesion molecule L1 like
• NCBI Official Symbol: CHL1
• NCBI Official Synonym Symbols: CALL; L1CAM2
• NCBI Protein Information: neural cell adhesion molecule L1-like protein
• UniProt Protein Name: Neural cell adhesion molecule L1-like protein
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: CHL1
• UniProt Synonym Gene Names: CALL

NCBI Description

The protein encoded by this gene is a member of the L1 gene family of neural cell adhesion molecules. It is a neural recognition molecule that may be involved in signal transduction pathways. The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome. This protein may also play a role in the growth of certain cancers. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Nov 2011]

Uniprot Description

CHL1: Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p26.3

Biological Process: signal transduction