Mouse anti-Human CD71 (Transferrin receptor) Monoclonal Antibody

Mouse Anti-Human CD71-APC

Cat. Num. AAA674283

• Reactivity: Human
• Applications: Flow Cytometry
• Synonyms: CD71 (Transferrin receptor); Monoclonal Antibody; Mouse Anti-Human CD71-APC; Mouse Anti-Human CD71 - Allophycocyanin; Mouse Anti-Human CD71 (Transferrin receptor); anti-CD71 (Transferrin receptor) antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: RVS10
• Specificity: Human CD71; CD71 is a 95 kDa type II heterodimeric transmembrane glycoprotein that is the receptor for transferrin. CD71 expression is very low on resting leukocytes but is upregulated upon activation. CD71 plays a critical role in cell proliferation by controlling the supply of iron, which is essential for many metabolic pathways, through the binding and endocytosis of transferrin.1-4
• Form/Format: Allophycocyanin (APC) Conjugate

• Applicable Applications for anti-CD71 (Transferrin receptor) antibody: Flow Cytometry
• Application Notes: Flow Cytometry Suggested Dilution: 10 uL/10^6 cells
• Characterization: To insure lot-to-lot consistency, each batch of product is tested by flow cytometry to conform with the characteristics of a standard reference reagent.
• Preparation and Storage: The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C. ; The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) and Allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.

References

1. Barclay, A.N., M.H. Brown, S.K.A. Law, A.J. McKnight, M.G. Tomlinson, and P.A. van der Merwe, eds. 1997. The Leukocyte Antigens Facts Book, 2nd Edition, CD71 Section, Academic Press, New York, p. 320
2. Tetsa, U., E. Pelosi, and C. Peschle 1993. Crit. Rev. Oncog. 4:241.
3. Trowbridge, I.S., J.F. Collawn, and C.R. Hopkins. 1993. Annu. Rev. Cell Biol. 9:129.
4. Hentze, M.W., and L.C. Kuhn. 1996. Proc. Natl. Acad. Sci. USA 93:8175.