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Testing Data
(Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70 )
Rat anti-Mouse CD70 Monoclonal Antibody | anti-CD70 antibody
Rat Anti Mouse CD70: RPE
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7; Tnlg8a
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD70; Monoclonal Antibody; Rat Anti Mouse CD70: RPE; CD70 antibody (FR70); anti-CD70 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
FR70
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Lyophilized
Sequence Length
195
Applicable Applications for anti-CD70 antibody
Flow Cytometry (FC/FACS)
Immunogen
A20 mouse B cell lymphoma cell line
Conjugation
RPE
Reconstitution
Reconstitute with 1.0 ml distilled water Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Pre-Lyophilization Buffer
Phosphate Buffered Saline
Fusion Partners
Spleen cells from immunized F344/DuCrj rats were fused with cells of the mouse P3U1 myeloma cell line.
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
1% Bovine Serum Albumin
5% Sucrose
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Testing Data
(Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70 )
Testing Data
(Staining of LPS activated mouse spleen cells with Rat anti Mouse CD70 )
Testing Data
(Staining of LPS activated mouse spleen cells with Alexa Fluor 647 conjugated Rat anti Mouse CD70: )
Testing Data
(Staining of LPS activated mouse spleen cells with Alexa Fluor 647 conjugated Rat anti Mouse CD70: )
Testing Data
(Staining of LPS activated mouse spleen cells with Alexa Fluor 488 conjugated Rat anti Mouse CD70 )
Testing Data
(Staining of LPS activated mouse spleen cells with Alexa Fluor 488 conjugated Rat anti Mouse CD70 )
Testing Data
(Staining of LPS stimulated mouse spleen cells with biotinylated Rat anti-Mouse CD70 )
Testing Data
(Staining of LPS stimulated mouse spleen cells with biotinylated Rat anti-Mouse CD70 )
Related Product Information for anti-CD70 antibody
Rat anti Mouse CD70 antibody, clone FR70 recognizes mouse CD70, a 30-33 kDa type II transmembrane protein belonging to the TNF superfamily. CD70 is not expressed on resting T cells and B cells but expression is induced on activation. Interaction of CD70 with its ligand CD27 is reported to play an important role in T cell co-stimulation and the regulation of T-dependant B cell activation and antibody production.
Clone FR70 is reported to block the functions of CD70 and has been used to block the interactions between CD27 and CD70 (Oshima et al.).
Clone FR70 is reported to block the functions of CD70 and has been used to block the interactions between CD27 and CD70 (Oshima et al.).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 antigen
NCBI Official Symbol
Cd70
NCBI Official Synonym Symbols
Cd27l; CD27LG; Tnfsf7; Tnlg8a
NCBI Protein Information
CD70 antigen
UniProt Protein Name
CD70 antigen
Protein Family
UniProt Gene Name
Cd70
UniProt Synonym Gene Names
Cd27l; Cd27lg; Tnfsf7; CD27-L
UniProt Entry Name
CD70_MOUSE
Uniprot Description
CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction
Research Articles on CD70
Similar Products
Product Notes
The CD70 cd70 (Catalog #AAA225654) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti Mouse CD70: RPE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD70 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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