Mouse anti-Human CD7 Monoclonal Antibody | anti-CD7 antibody

Anti-CD7 Antibody, Mouse Monoclonal

Cat. Num. AAA8104637

• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD7; Monoclonal Antibody; Anti-CD7 Antibody; Mouse Monoclonal; CD7 Antibody; Mouse MAb; CD7 molecule; Anti-GP40 Antibody; Anti-LEU-9 Antibody; Anti-Tp40 Antibody; Anti-TP41 Antibody; anti-CD7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG2b
• Clone Number: 21
• Specificity: Human CD7
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD7 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:30-1:100; FC/FACS/FCM: 1:25-1:100
• Immunogen: Human CD7 Plasmid
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with Human CD7 Plasmid. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CD7 mouse monoclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:25 kDa Observed band size:37 kDa)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD7 expression on human whole blood Lymphocytes. Cells were stained with purified anti-Human CD7, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable Lymphocytes.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD7 in human lymph node with mouse monoclonal antibody at 1:60 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-CD7 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD7 is a transmembrane protein that is a member of the immunoglobulin superfamily. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.