anti-Mouse CD69 (VEA) Monoclonal Antibody | anti-Cd69 antibody

Hamster Anti-Mouse CD69/VEA-LE/AF

Cat. Num. AAA673432

• Gene Names: Cd69; AIM; VEA; AI452015; 5830438K24Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD69 (VEA); Monoclonal Antibody; Hamster Anti-Mouse CD69/VEA-LE/AF; Hamster Anti-Mouse CD69/VEA - Low Endotoxin/Azide-Free; Hamster Anti-Mouse CD69 (VEA); anti-Cd69 antibody

• Host: Armenian Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Armenian Hamster IgG lambda
• Clone Number: H1.2F3
• Specificity: Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced); CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa.^1,2 It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules.^3 CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils.^1-4 Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.^4,5 MAb H1.2F3 augments PMA-induced T-cell proliferation,^1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells.^7
• Form/Format: Low Endotoxin, Azide - Free (LE/AF)
• Sequence Length: 199

• Applicable Applications for anti-Cd69 antibody: Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
• Application Notes: Research Application(s): In vitro T cell and NK-cell activation
• Immunogen: Y245 mouse dendritic epidermal T cell line
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Yokoyama, W. M., F. Koning, P. J. Kehn, G. M. Pereira, G. Stingl, J. E. Coligan, and E. M. Shevach. 1988. J. Immunol. 141:369.
2. Yokoyama, W.M., S.R. Maxfield, and E.M. Shevach. 1989. Immunol. Rev. 109:153.
3. Zeigler, S.F., S.D. Levin, L. Johnson, N.G. Copeland, et al. 1994. J. Immunol. 152:1228.
4. Ziegler, S.F., F. Ramsdell, and M.R. Alderson. 1994. Stem Cells 12:456.
5. Brandle, D., S. Muller, C. Muller, H. Hengartner, and H. Pircher. 1994. Eur. J. Immunol. 24:145.
6. Sobel, E. S., W. M. Yokoyama, E. M. Shevach, R. A. Eisenberg, and P. L. Cohen. 1993. J. Immunol. 150:673.
7. Karlhofer, F. M. and W. M. Yokoyama. 1991. J. Immunol. 146:3662.

NCBI and Uniprot Product Information

• NCBI GI #: 187954213
• NCBI GeneID: 12515
• UniProt Accession #: P37217; Q3U6A8
• NCBI Official Full Name: CD69 antigen
• NCBI Official Synonym Full Names: CD69 antigen
• NCBI Official Symbol: Cd69
• NCBI Official Synonym Symbols: AIM; VEA; AI452015; 5830438K24Rik
• NCBI Protein Information: early activation antigen CD69; Very Early Activation Antigen
• UniProt Protein Name: Early activation antigen CD69
• Protein Family: Early activation antigen
• UniProt Entry Name: CD69_MOUSE

Uniprot Description

CD69: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.

Protein type: Cell surface; Membrane protein, integral

Cellular Component: membrane; integral to membrane; external side of plasma membrane

Molecular Function: calcium ion binding; carbohydrate binding