Rabbit anti-Human IL5RA Polyclonal Antibody | anti-IL5RA antibody

IL5RA, NT (IL5RA, IL5R, Interleukin-5 receptor subunit alpha, CDw125, CD125) (FITC)

Cat. Num. AAA6310281

• Gene Names: IL5RA; IL5R; CD125; CDw125; HSIL5R3
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IL5RA; Polyclonal Antibody; NT (IL5RA; IL5R; Interleukin-5 receptor subunit alpha; CDw125; CD125) (FITC); CD125; anti-IL5RA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognies human IL5RA.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-IL5RA antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), FLISA, Flow Cytometry (FC/FACS)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 49-76 in the N-terminal region of human IL5RA, UniProt Accession #Q01344.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of MDA-MB-453 cell line,lysate (35ug/ml) using MBS6013593 at 1:1000.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human lung carcinoma using MBS6013593 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of U251 cells using MBS6013593 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-IL5RA antibody

Antibodies; CD Markers

References

Matarin, M., et al. Stroke 40(11):3436-3442(2009). Beekman, J.M., et al. Blood 114(18):3917-3927(2009). Song, X.Y., et al. Diabetologia 52(8):1543-1553(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 191175130
• NCBI GeneID: 3568
• UniProt Accession #: Q01344; Q14633; Q15469; Q6ISX9; B3IU77; B4E2G0
• Molecular Weight: 47,685 Da
• NCBI Official Full Name: interleukin 5 receptor alpha transcript variant 7
• NCBI Official Synonym Full Names: interleukin 5 receptor, alpha
• NCBI Official Symbol: IL5RA
• NCBI Official Synonym Symbols: IL5R; CD125; CDw125; HSIL5R3
• NCBI Protein Information: interleukin-5 receptor subunit alpha; CD125 antigen; IL-5R subunit alpha; IL-5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain
• UniProt Protein Name: Interleukin-5 receptor subunit alpha
• Protein Family: Interleukin-5 receptor
• UniProt Gene Name: IL5RA
• UniProt Synonym Gene Names: IL5R; IL-5 receptor subunit alpha; IL-5R subunit alpha; IL-5R-alpha; IL-5RA
• UniProt Entry Name: IL5RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 5 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL5 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL5. This protein has been found to interact with syndecan binding protein (syntenin), which is required for IL5 mediated activation of the transcription factor SOX4. Several alternatively spliced transcript variants encoding four distinct isoforms have been reported. [provided by RefSeq, Jul 2011]

Uniprot Description

IL5RA: This is the receptor for interleukin-5. The alpha chain binds to IL5. Belongs to the type I cytokine receptor family. Type 5 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 3p26-p24

Cellular Component: extracellular space; plasma membrane; integral to membrane

Molecular Function: protein binding; interleukin-5 receptor activity

Biological Process: cell proliferation; regulation of interleukin-5 production; inflammatory response to antigenic stimulus; signal transduction