anti-Mouse CD69 (VEA) Monoclonal Antibody | anti-Cd69 antibody
Hamster Anti-Mouse CD69/VEA-APC
Gene Names
Cd69; AIM; VEA; AI452015; 5830438K24Rik
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD69 (VEA); Monoclonal Antibody; Hamster Anti-Mouse CD69/VEA-APC; Hamster Anti-Mouse CD69/VEA - Allophycocyanin; Hamster Anti-Mouse CD69 (VEA); anti-Cd69 antibody
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Armenian Hamster IgG lambda
Clone Number
H1.2F3
Specificity
Mouse CD69/Very Early Activation Antigen (VEA), Mr 85 kDa (unreduced)
CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa.^1,2 It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules.^3 CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils.^1-4 Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.^4,5 MAb H1.2F3 augments PMA-induced T-cell proliferation,^1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells.^7
CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa.^1,2 It is a C-type lectin, most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules.^3 CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils.^1-4 Although not detectable on resting lymphocytes, its expression is rapidly (within 2 hours) upregulated upon activation of T, B and NK cells, and neutrophils.4 Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells.^4,5 MAb H1.2F3 augments PMA-induced T-cell proliferation,^1,6 and induces redirected lysis of Fc receptor-bearing target cells by NK cells.^7
Form/Format
Allophycocyanin (APC) Conjugate
Applicable Applications for anti-Cd69 antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In vitro T cell and NK-cell activation
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Immunogen
Y245 mouse dendritic epidermal T cell line
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Yokoyama, W. M., F. Koning, P. J. Kehn, G. M. Pereira, G. Stingl, J. E. Coligan, and E. M. Shevach. 1988. J. Immunol. 141:369.
2. Yokoyama, W.M., S.R. Maxfield, and E.M. Shevach. 1989. Immunol. Rev. 109:153.
3. Zeigler, S.F., S.D. Levin, L. Johnson, N.G. Copeland, et al. 1994. J. Immunol. 152:1228.
4. Ziegler, S.F., F. Ramsdell, and M.R. Alderson. 1994. Stem Cells 12:456.
5. Brandle, D., S. Muller, C. Muller, H. Hengartner, and H. Pircher. 1994. Eur. J. Immunol. 24:145.
6. Sobel, E. S., W. M. Yokoyama, E. M. Shevach, R. A. Eisenberg, and P. L. Cohen. 1993. J. Immunol. 150:673.
7. Karlhofer, F. M. and W. M. Yokoyama. 1991. J. Immunol. 146:3662.
2. Yokoyama, W.M., S.R. Maxfield, and E.M. Shevach. 1989. Immunol. Rev. 109:153.
3. Zeigler, S.F., S.D. Levin, L. Johnson, N.G. Copeland, et al. 1994. J. Immunol. 152:1228.
4. Ziegler, S.F., F. Ramsdell, and M.R. Alderson. 1994. Stem Cells 12:456.
5. Brandle, D., S. Muller, C. Muller, H. Hengartner, and H. Pircher. 1994. Eur. J. Immunol. 24:145.
6. Sobel, E. S., W. M. Yokoyama, E. M. Shevach, R. A. Eisenberg, and P. L. Cohen. 1993. J. Immunol. 150:673.
7. Karlhofer, F. M. and W. M. Yokoyama. 1991. J. Immunol. 146:3662.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD69 antigen
NCBI Official Synonym Full Names
CD69 antigen
NCBI Official Symbol
Cd69
NCBI Official Synonym Symbols
AIM; VEA; AI452015; 5830438K24Rik
NCBI Protein Information
early activation antigen CD69; Very Early Activation Antigen
UniProt Protein Name
Early activation antigen CD69
Protein Family
UniProt Entry Name
CD69_MOUSE
Uniprot Description
CD69: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets.
Protein type: Cell surface; Membrane protein, integral
Cellular Component: membrane; integral to membrane; external side of plasma membrane
Molecular Function: calcium ion binding; carbohydrate binding
Research Articles on Cd69
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Product Notes
The Cd69 (Catalog #AAA673430) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD69/VEA-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 (VEA) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation. Research Application(s): In vitro T cell and NK-cell activation Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the Cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69 (VEA), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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