Testing Data
Mouse anti-Human CD69 Monoclonal Antibody | anti-CD69 antibody
anti-human CD69-Biotin
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD69; Monoclonal Antibody; anti-human CD69-Biotin; Monoclonal anti-human CD69(AIM)/Biotin; anti-CD69 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
HP-4B3
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
21
Applicable Applications for anti-CD69 antibody
Flow Cytometry (FC/FACS)
Performance
Five x 105 cultured HEL92.1 cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD69/Biotin at 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.06 log10 fluorescent units when compared to a Mouse IgG2a/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 mul of 0.5 mg/ml anti-CD69 antibody.
Conjugation
Biotin
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen
IL-2 activated human NK cells
Preparation and Storage
Store at 2 - 5 degree C. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD69 antibody
Information: Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Antibody HP-4B3 recognizes the dimeric CD69 molecule of 60 kd.
References
S.F. Ziegler, et al, Stem Cells (1994) 12: 456-465. R. Testi, et al, Immunol Today (1994) 15: 479-483. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 1123-1132. D. Vanhecke, et al, J Immunol (1995) 155: 1862-1872.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
2,282 Da
NCBI Official Full Name
CD69, partial
UniProt Protein Name
CD69 protein
Protein Family
UniProt Gene Name
CD69
UniProt Entry Name
Q6LAB3_HUMAN
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Product Notes
The CD69 cd69 (Catalog #AAA666058) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD69-Biotin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD69 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD69, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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