CD68 Monoclonal Antibody | anti-CD68 antibody

CD68 Mouse Monoclonal Antibody

Cat. Num. AAA8500868

• Gene Names: CD68; GP110; LAMP4; SCARD1
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry
• Synonyms: CD68; Monoclonal Antibody; CD68 Mouse Monoclonal Antibody; anti-CD68 antibody

• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Clone Number: 6F3
• Specificity: The CD68 antibody detects endogenous CD68 proteins (~37KD).
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 354

• Applicable Applications for anti-CD68 antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:200
• Preparation and Storage: Store at-20 degree C for 1 year

Testing Data

(IHC staining of human tonsil tissue CD68 mouse mAb(6F3) diluted at 1:200.)

Related Product Information for anti-CD68 antibody

The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue-and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.

NCBI and Uniprot Product Information

• NCBI GI #: 298665
• NCBI GeneID: 968
• Molecular Weight: 34,654 Da
• NCBI Official Full Name: CD68
• NCBI Official Synonym Full Names: CD68 molecule
• NCBI Official Symbol: CD68
• NCBI Official Synonym Symbols: GP110; LAMP4; SCARD1
• NCBI Protein Information: macrosialin
• UniProt Protein Name: Macrosialin
• Protein Family: Macrosialin
• UniProt Gene Name: CD68

NCBI Description

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.CautionCD68 is a commonly used marker for macrophages. However, a number of studies (PubMed:15194571, PubMed:15647451 and PubMed:18405323) have shown that CD68 antibodies react with other hematopoietic and non-hematopoietic cell types, suggesting that CD68 may not be a macrophage-specific antigen.