Rabbit CYP3A4 Polyclonal Antibody | anti-CYP3A4 antibody

CYP3A4 Rabbit Polyclonal

Cat. Num. AAA768397

• Gene Names: CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: CYP3A4; Polyclonal Antibody; CYP3A4 Rabbit Polyclonal; anti-CYP3A4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 502

• Applicable Applications for anti-CYP3A4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Immunogen: Cytochrome P450, family 3, subfamily A, polypeptide 4
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver using MBS768397 (CYP3A4 antibody) at dilution of 1:50)

Immunofluorescence (IF)

(Immunofluorescent analysis of (-20 degree C Ethanol) fixed HepG2 cells using MBS768397 (CYP3A4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L))

Western Blot (WB)

(rat liver tissue were subjected to SDS PAGE followed by western blot with MBS768397 (CYP3A4 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 322302351
• NCBI GeneID: 1576
• NCBI Accession #: NP_001189784.1
• NCBI GenBank Nucleotide #: NM_001202855.2
• Molecular Weight: 57,343 Da
• NCBI Official Full Name: cytochrome P450 3A4 isoform 2
• NCBI Official Synonym Full Names: cytochrome P450 family 3 subfamily A member 4
• NCBI Official Symbol: CYP3A4
• NCBI Official Synonym Symbols: HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1
• NCBI Protein Information: cytochrome P450 3A4
• UniProt Protein Name: Cytochrome P450 3A4
• Protein Family: Cytochrome
• UniProt Gene Name: CYP3A4
• UniProt Synonym Gene Names: CYP3A3
• UniProt Entry Name: CP3A4_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam and erythromycin. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Feb 2011]

Uniprot Description

CYP3A4: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4- hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2- exo-monooxygenase. The enzyme also hydroxylates etoposide. Belongs to the cytochrome P450 family.

Protein type: Membrane protein, integral; Lipid Metabolism - linoleic acid; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.14.14.1; Cell surface; Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: cytoplasm; endoplasmic reticulum membrane; integral to membrane; intracellular membrane-bound organelle

Molecular Function: albendazole monooxygenase activity; enzyme binding; heme binding; iron ion binding; monooxygenase activity; oxidoreductase activity; oxygen binding; quinine 3-monooxygenase activity; steroid binding; steroid hydroxylase activity; taurochenodeoxycholate 6alpha-hydroxylase activity; testosterone 6-beta-hydroxylase activity; vitamin D3 25-hydroxylase activity

Biological Process: alkaloid catabolic process; androgen metabolic process; drug catabolic process; drug metabolic process; exogenous drug catabolic process; heterocycle metabolic process; lipid metabolic process; monoterpenoid metabolic process; steroid catabolic process; steroid metabolic process; vitamin D metabolic process; xenobiotic metabolic process