Mouse anti-Human, Mouse CD63 Monoclonal Antibody | anti-CD63 antibody

CD63 (gp55, granulophysin, Lysosomal-associated membrane protein 3 (LAMP-3), Mast cell antigen AD1, melanoma 1 antigen, Melanoma-associated antigen MLA1, Melanoma-associated antigen ME491, MLA1, NGA, Ocular melanoma-associated antigen, OMA81H, PTLGP40, Te

Cat. Num. AAA6122859

• Gene Names: CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: CD63; Monoclonal Antibody; CD63 (gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Te; gp55; Tetraspanin-30; TSPAN30; anti-CD63 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: MX-49.129.5
• Specificity: Recognizes human CD63. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-CD63 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.; Applications are based on unconjugated antibody.
• Immunogen: Full length CD63 of human origin. Cellular Localization: Cytoplasmic and cell surface
• Conjugate: HRP
• Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of human Spleen Lysate using 168713-AF.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human Melanoma stained with 168713-AF.)

Product Categories/Family for anti-CD63 antibody

Antibodies; CD Markers

References

1. C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985. 2. AA Palmer et al., Pathology 17: 335-339, 1985. 3. EC Hagen et al., Histopathology 10: 689-700, 1986.

NCBI and Uniprot Product Information

• NCBI GI #: 189384
• NCBI GeneID: 967
• UniProt Accession #: P08962; Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
• Molecular Weight: 23,430 Da
• NCBI Official Full Name: melanoma-associated antigen
• NCBI Official Synonym Full Names: CD63 molecule
• NCBI Official Symbol: CD63
• NCBI Official Synonym Symbols: MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• NCBI Protein Information: CD63 antigen
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: CD63
• UniProt Synonym Gene Names: MLA1; TSPAN30; LAMP-3; Tspan-30

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.