Mouse CD62P Monoclonal Antibody | anti-CD62P antibody

MOUSE ANTI HUMAN CD62P:RPE

Cat. Num. AAA224665

• Gene Names: SELP; CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD62P; Monoclonal Antibody; MOUSE ANTI HUMAN CD62P:RPE; anti-CD62P antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: AK-6
• Form/Format: Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
• Sequence Length: 830

• Applicable Applications for anti-CD62P antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Maximum Dilution: Neat
• Reconstitution: Reconstitute with 1 ml distilled water; Perservative Stabilisers
• Immunogen: Human platelet membrane glycoproteins; Buffer Solution
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of human tonsil showing capillary endothelium. Formalin fixed, paraffin processed tissue with Mouse anti Human CD62P (P-Selectin) (MBS224591))

Testing Data #2

(Staining of human peripheral blood platelets with Mouse anti Human CD62P (MBS224591))

Testing Data #3

(Staining of thrombin activated human peripheral blood platelets with Mouse anti Human CD62P: FITC (MBS224428))

Testing Data #4

(Staining of thrombin activated human platelets with Mouse anti Human CD62P:Azide Free (MBS224694))

Testing Data #5

(Staining of thrombin activated platelets with Mouse anti Human CD62P (MBS224420))

Related Product Information for anti-CD62P antibody

Mouse anti Human CD62P antibody, clone AK-6 recognizes the CD62P glycoprotein, a 140kD molecule expressed by activated platelets and endothelial cells. CD62P is also known as P-selectin, PADGEM or GMP140 and plays an important role in adhesive processes between leucocytes and endothelial cells. CD62P is a component of the alpha granule and is translocated to the plasma membrane upon activation.

NCBI and Uniprot Product Information

• NCBI GI #: 157419154
• NCBI GeneID: 6403
• NCBI Accession #: NP_002996.2
• NCBI GenBank Nucleotide #: NM_003005.3
• UniProt Accession #: P16109; Q5R344; Q8IVD1
• Molecular Weight: 90,834 Da
• NCBI Official Full Name: P-selectin
• NCBI Official Synonym Full Names: selectin P (granule membrane protein 140kDa, antigen CD62)
• NCBI Official Symbol: SELP
• NCBI Official Synonym Symbols: CD62; GRMP; PSEL; CD62P; GMP140; LECAM3; PADGEM
• NCBI Protein Information: P-selectin; CD62 antigen-like family member P; GMP-140; granule membrane protein 140; granulocyte membrane protein; leukocyte-endothelial cell adhesion molecule 3; platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein
• UniProt Protein Name: P-selectin
• UniProt Gene Name: SELP
• UniProt Synonym Gene Names: GMRP; GRMP; GMP-140; LECAM3; PADGEM
• UniProt Entry Name: LYAM3_HUMAN

NCBI Description

This gene encodes a 140 kDa protein that is stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. This protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. The membrane protein is a calcium-dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens on neutrophils and monocytes. Alternative splice variants may occur but are not well documented. [provided by RefSeq, Jul 2008]

Uniprot Description

SELP: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the selectin/LECAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q22-q25

Cellular Component: nucleoplasm; extracellular space; platelet alpha granule membrane; integral to plasma membrane; cytoplasm; plasma membrane; platelet dense granule membrane; external side of plasma membrane

Molecular Function: heparin binding; protein binding; lipopolysaccharide binding; glycoprotein binding; glycosphingolipid binding; calcium-dependent protein binding; sialic acid binding; fucose binding

Biological Process: platelet activation; positive regulation of leukocyte migration; response to lipopolysaccharide; defense response to Gram-negative bacterium; heterophilic cell adhesion; positive regulation of phosphoinositide 3-kinase cascade; leukocyte adhesion; platelet degranulation; regulation of integrin activation; leukocyte tethering or rolling; inflammatory response; cell adhesion; blood coagulation; leukocyte migration

Disease: Ige Responsiveness, Atopic