Rat anti-Mouse CD62L (L-Selectin) Monoclonal Antibody
Rat Anti-Mouse CD62L/L-selectin-SPRD
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD62L (L-Selectin); Monoclonal Antibody; Rat Anti-Mouse CD62L/L-selectin-SPRD; Rat Anti-Mouse CD62L/L-selectin - SpectralRed-Labeled; Rat Anti-Mouse CD62L (L-Selectin); anti-CD62L (L-Selectin) antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat (Fisher) IgG2a kappa
Clone Number
MEL-14
Specificity
CD62L/L-selectin, Mr 90 kDa
CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.^1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.^1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.^3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.^1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.^10-13 The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.^1, 2, 7-9
CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and Epidermal Growth Factor-like domains.^1, 2 It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes.^1, 2 In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes and eosinophils.^3-7 This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation.^1, 2, 7-9 Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells.^10-13 The monoclonal antibody MEL-14 blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.^1, 2, 7-9
Form/Format
Spectral Red (SPRD) Conjugate
Applicable Applications for anti-CD62L (L-Selectin) antibody
Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation
Application Notes
Research Application(s): In vivo and in vitro blocking of adhesion
Cytotoxicity studies
Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells
Cytotoxicity studies
Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells
Immunogen
C3H/eb cloned mouse B lymphoma 38C-13
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and allophycocyanin-Cyanine 7 (APCCY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Gallatin, W.M., I.L. Weissman, and E.C. Butcher. 1983. Nature 304:30.
2. Siegelman, M.H., I.C. Cheng,, I.L. Weissman, and E.K. Wakeland. 1990. Cell 61:611.
3. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3521.
4. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3529.
5. Reichert, R.A., L. Jerabek, W.M. Gallatin, E.C. Butcher, and I.L. Weissman. 1986. J. Immunol. 136:3535.
6. Iwabuchi, K., J. Ohgama, K. Ogasawara, C. Iwabuchi, I. Negishi, R.A. Good, and K. Onoe. 1991. Immunobiology 182:161.
7. Lewinsohn, D.M., R.F. bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313.
8. Pizcueta, P., and F.W. Luscinskas. 1994. Am. J. Pathol. 145:461.
9. Ley, K., D.C. Bullard, M.L. Arbones, R. Bosse, D. Vestweber, T.F. Tedder, and A.L. Beaudet. 1995. J. Exp. Med. 181:669.
10. Jung, T.M., W.M. Gallatin, I.L. Weissman, and M.O. Dailey. 1988. J. Immunol. 141:4110.
11. Kishimoto, T.K., M.A. Jutila, E.L. berg, and E.C. Butcher. 1989. Science 245:1238.
12. Mobley, J.L., and M.O. Dailey. 1992. J. Immunol. 148:2348.
13. Sprent, J., and D.F. Tough. 1994. Science 265:1395.
2. Siegelman, M.H., I.C. Cheng,, I.L. Weissman, and E.K. Wakeland. 1990. Cell 61:611.
3. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3521.
4. Reichert, R.A., I.L. Weissman, and E.C. Butcher. 1986. J. Immunol. 136:3529.
5. Reichert, R.A., L. Jerabek, W.M. Gallatin, E.C. Butcher, and I.L. Weissman. 1986. J. Immunol. 136:3535.
6. Iwabuchi, K., J. Ohgama, K. Ogasawara, C. Iwabuchi, I. Negishi, R.A. Good, and K. Onoe. 1991. Immunobiology 182:161.
7. Lewinsohn, D.M., R.F. bargatze, and E.C. Butcher. 1987. J. Immunol. 138:4313.
8. Pizcueta, P., and F.W. Luscinskas. 1994. Am. J. Pathol. 145:461.
9. Ley, K., D.C. Bullard, M.L. Arbones, R. Bosse, D. Vestweber, T.F. Tedder, and A.L. Beaudet. 1995. J. Exp. Med. 181:669.
10. Jung, T.M., W.M. Gallatin, I.L. Weissman, and M.O. Dailey. 1988. J. Immunol. 141:4110.
11. Kishimoto, T.K., M.A. Jutila, E.L. berg, and E.C. Butcher. 1989. Science 245:1238.
12. Mobley, J.L., and M.O. Dailey. 1992. J. Immunol. 148:2348.
13. Sprent, J., and D.F. Tough. 1994. Science 265:1395.
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Product Notes
The CD62L (L-Selectin) (Catalog #AAA673421) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD62L/L-selectin-SPRD reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD62L (L-Selectin) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation. Research Application(s): In vivo and in vitro blocking of adhesion Cytotoxicity studies Flow Cytometry Suggested Dilution: <= 0.1 ug/10^6 cells. Researchers should empirically determine the suitability of the CD62L (L-Selectin) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD62L (L-Selectin), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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