Mouse anti-Human, Mouse CD6 Monoclonal Antibody | anti-CD6 antibody

CD6 Mouse Monoclonal Antibody

Cat. Num. AAA438533

• Gene Names: CD6; TP120
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD6; Monoclonal Antibody; CD6 Mouse Monoclonal Antibody; T12; TP120; anti-CD6 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: SPV-L14
• Specificity: CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular -scavenger receptor cysteine-rich- (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 shows significant homology to CD5. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 601

• Applicable Applications for anti-CD6 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml)
• Cellular Localization: Cell Surface and Cytoplasmic
• Immunogen: CD8+ cytotoxic T-cell clone
• Hu-Chromosome Location: 11q12.2
• Positive Control: CCRF-CEM, Jurkat cells, Tonsil
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

Kamoun et al., J. Immunol. 127, 987, 1981 Reinherz et al., Proc. Nat. Acad. Sci. 79, 6047, 1982 Yssel, H., de Vries, J.E., Borst, J. and Spits, H. Distribution and functional analysis of a 120-130 kDa T cell surface antigen. Cell. Immunol. 105: 161-173, 1987. Bazil, V et. al. 1989. Monoclonal antibodies against human leucocyte antigens. III. Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM-53 and MEM-102. Folia. Biol. 35:289-297

NCBI and Uniprot Product Information

• NCBI GI #: 363543322
• NCBI GeneID: 923
• NCBI Accession #: NP_001241679.1
• NCBI GenBank Nucleotide #: NM_001254750.1
• UniProt Accession #: P30203; Q9UMF2; Q9Y4K7; Q9Y4K8; Q9Y4K9; Q9Y4L0; A4KAD4; A4KAD5
• Molecular Weight: 90-130kDa
• NCBI Official Full Name: T-cell differentiation antigen CD6 isoform 2
• NCBI Official Synonym Full Names: CD6 molecule
• NCBI Official Symbol: CD6
• NCBI Official Synonym Symbols: TP120
• NCBI Protein Information: T-cell differentiation antigen CD6
• UniProt Protein Name: T-cell differentiation antigen CD6
• Protein Family: CD63 antigen
• UniProt Gene Name: CD6
• UniProt Entry Name: CD6_HUMAN

NCBI Description

This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis (PMID: 19525953, 21849685). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CD6: a type I membrane protein involved in cell adhesion. Binds to CD166. Expressed on thymocytes, peripheral T cells, a subset of B cells, and a subset of neurons. Mediates the binding of developing thymocytes with thymic epithelial cells. CD6- T cells are less autoreactive than CD6+ T cells. Phosphorylated on tyrosines and serines following T cell antigen receptor signaling. Five splice-variant isoforms have been described.

Protein type: Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: immunological synapse; integral to plasma membrane; intrinsic to plasma membrane; T cell receptor complex

Molecular Function: lipopolysaccharide binding; protein binding

Biological Process: acute inflammatory response to antigenic stimulus; formation of immunological synapse; heterophilic cell adhesion; lipopolysaccharide-mediated signaling pathway; positive regulation of T cell proliferation; response to lipopolysaccharide