Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD6 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Rat CD6 Monoclonal Antibody | anti-CD6 antibody
Anti-CD6 Antibody, Mouse Monoclonal
Gene Names
Cd6; OX52
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD6; Monoclonal Antibody; Anti-CD6 Antibody; Mouse Monoclonal; CD6/Cluster of Differentiation 6 Antibody; Mouse MAb; CD6 molecule; anti-CD6 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG2a
Clone Number
01
Specificity
Rat CD6/Cluster of Differentiation 6
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD6 antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Rat CD6/Cluster of Differentiation 6 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD6/Cluster of Differentiation 6 (rR CD6/Cluster of Differentiation 6). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD6 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD6 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CD6 antibody
T-cell differentiation antigen CD6, also known as TP12 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6/TP12 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6/TP12 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6/TP12 binds to activated leukocyte cell adhesion molecule (CD166), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6/TP12 partially associates with the TCR/CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.
Product Categories/Family for anti-CD6 antibody
References
Joo YS. et al., 2000, Arthritis Rheum. 43 (2): 329-35. Singer NG. et al., 2002, Int Immunol. 14 (6): 585-97. Gimferrer I. et al., 2005, J Immunol. 175 (3): 1406-14. Alonso R. et al., 2010, J Autoimmun. 35 (4): 336-41.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
T-cell differentiation antigen CD6
NCBI Official Synonym Full Names
Cd6 molecule
NCBI Official Symbol
Cd6
NCBI Official Synonym Symbols
OX52
NCBI Protein Information
T-cell differentiation antigen CD6
UniProt Protein Name
CD6 antigen
Protein Family
UniProt Gene Name
Cd6
UniProt Entry Name
Q812A4_RAT
NCBI Description
cell surface molecule found on T lymphocytes that may regulate CD5 tyrosine phosphorylation; associates with CD5, Lck, Fyn, ZAP-70 and Itk [RGD, Feb 2006]
Research Articles on CD6
Similar Products
Product Notes
The CD6 cd6 (Catalog #AAA8112930) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD6 Antibody, Mouse Monoclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD6 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CD6 cd6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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