Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27 (MBS488736) Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 193-27 (MBS488737) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488737, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Mouse anti-Human CD59 Monoclonal Antibody | anti-CD59 antibody
Anti-CD59 [193-27]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27 (MBS488736) Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 193-27 (MBS488737) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488737, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD59 antibody 193-27 (MBS488736) Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 193-27 (MBS488737) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488737, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-CD59 antibody 193-27 (MBS488736). Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of 193-27 (MBS488737, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Flow Cytometry (FC/FACS)
(Flow cytometry using the anti-CD59 antibody 193-27 (MBS488736). Jurkat cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG1 version of 193-27 (MBS488737, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
This antibody was raised by immunizng mice with stimulated human leukocytes.
Note on publication: This article describes the use of the antibody 161-2.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
Protein type: Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 11p13
Cellular Component: anchored to external side of plasma membrane; compact myelin; extracellular space; cell surface; focal adhesion; membrane; extracellular region; plasma membrane; sarcolemma; vesicle
Molecular Function: complement binding; protein binding
Biological Process: cell activation; cell surface receptor linked signal transduction; regulation of complement activation; innate immune response; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; blood coagulation; negative regulation of apoptosis
Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy
Research Articles on CD59
Similar Products
Product Notes
The CD59 cd59 (Catalog #AAA488736) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD59 [193-27] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD59 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunofluorescence (IF). The antibody 193-27 is recommended for the identification and analysis of CD59 via various experimental techniques (Bellosillo et al., 2001). Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH) and thus this antibody is useful for study on GPI-anchored proteins, PNH and CD59 functions. Researchers should empirically determine the suitability of the CD59 cd59 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD59, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
