Rabbit anti-Human CD59 Monoclonal Antibody | anti-CD59 antibody

Recombinant Anti-CD59 Antibody (PerCP), Rabbit Monoclonal

Cat. Num. AAA8106470

• Gene Names: CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: CD59; Monoclonal Antibody; Recombinant Anti-CD59 Antibody (PerCP); Rabbit Monoclonal; CD59 Antibody (PerCP); Rabbit MAb; CD59 molecule; Anti-16.3A5 Antibody; Anti-1F5 Antibody; Anti-EJ16 Antibody; Anti-EJ30 Antibody; Anti-EL32 Antibody; Anti-FLJ38134 Antibody; Anti-FLJ92039 Antibody; Anti-G344 Antibody; Anti-HRF-20 Antibody; Anti-HRF20 Antibody; Anti-MAC-IP Antibody; Anti-MACIF Antibody; Anti-MEM43 Antibody; Anti-MGC2354 Antibody; Anti-MIC11 Antibody; Anti-MIN1 Antibody; Anti-MIN2 Antibody; Anti-MIN3 Antibody; Anti-MIRL Antibody; Anti-MSK21 Antibody; Anti-p18-20 Antibody; anti-CD59 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 029
• Specificity: Human CD59
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-CD59 antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human CD59 Protein
• Conjugation: PerCP
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD59 (rh CD59) and conjugated with PerCP under optimum conditions, the unreacted PerCP was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD59 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD59. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human CD59 expression on human whole blood lymphocytes. Cells were stained with PerCP-conjugated anti-Human CD59. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

Related Product Information for anti-CD59 antibody

CD59 glycoprotein, also known as 2 kDa homologous restriction factor, HRF2, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Product Categories/Family for anti-CD59 antibody

Complement Activation Pathways

References

Fletcher CM. et al., 1994, Structure. 2: 185-99. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9. Picariello G. et al., 2008, Proteomics 8: 3833-47. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.

NCBI and Uniprot Product Information

• NCBI GI #: 42716299
• NCBI GeneID: 966
• NCBI Accession #: NP_976076.1
• NCBI GenBank Nucleotide #: NM_203331.2
• UniProt Accession #: P13987
• Molecular Weight: 14,177 Da
• NCBI Official Full Name: CD59 glycoprotein preproprotein
• NCBI Official Synonym Full Names: CD59 molecule, complement regulatory protein
• NCBI Official Symbol: CD59
• NCBI Official Synonym Symbols: 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
• NCBI Protein Information: CD59 glycoprotein; protectin; 1F5 antigen; MEM43 antigen; Ly-6-like protein; T cell-activating protein; human leukocyte antigen MIC11; lymphocytic antigen CD59/MEM43; 20 kDa homologous restriction factor; membrane inhibitor of reactive lysis; membrane att
• UniProt Protein Name: CD59 glycoprotein
• Protein Family: CD59 glycoprotein
• UniProt Gene Name: CD59
• UniProt Synonym Gene Names: MIC11; MIN1; MIN2; MIN3; MSK21; HRF-20; HRF20; MAC-IP; MACIF; MIRL
• UniProt Entry Name: CD59_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. This protein also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Multiple alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CD59: Potent inhibitor of the complement membrane attack complex (MAC) action. Acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. Involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: anchored to external side of plasma membrane; compact myelin; extracellular space; cell surface; focal adhesion; membrane; extracellular region; plasma membrane; sarcolemma; vesicle

Molecular Function: complement binding; protein binding

Biological Process: cell activation; cell surface receptor linked signal transduction; regulation of complement activation; innate immune response; negative regulation of activation of membrane attack complex; positive regulation of T cell proliferation; blood coagulation; negative regulation of apoptosis

Disease: Hemolytic Anemia, Cd59-mediated, With Or Without Immune-mediated Polyneuropathy