Mouse anti-Human, Rat CD56/NCAM1/NKH1 Monoclonal Antibody | anti-NCAM1 antibody

CD56/NCAM1/NKH1 (Neuronal Cell Marker)

Cat. Num. AAA4381111

• Gene Names: NCAM1; CD56; NCAM; MSK39
• Reactivity: Human, Rat
• Applications: Immunohistochemistry
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD56/NCAM1/NKH1; Monoclonal Antibody; CD56/NCAM1/NKH1 (Neuronal Cell Marker); NCAM; Leu-19; NKH1; MSK39; NCAM120; NCAM140; NCAM180; Neural Cell Adhesion Molecule; anti-NCAM1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: ERIC-1
• Specificity: This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the nodal and nasal NK/T-cell lymphomas.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 858

• Applicable Applications for anti-NCAM1 antibody: Immunohistochemistry (IHC) Paraffin
• Application Notes: Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Positive Control: Cerebellum, Pancreas, Neuroblastoma.
• Immunogen: Recombinant human NCAM1 protein
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 11q23.1
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with CD56 Mouse Monoclonal Antibody (ERIC-1).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon stained with CD56 Mouse Monoclonal Antibody (ERIC-1).)

SDS-Page

(SDS-PAGE Analysis Purified CD56 Mouse Monoclonal Antibody (ERIC-1). Confirmation of Purity and Integrity of Antibody.)

References

Endo, C., et al. 2009. Acta Cytol. 53: 36-40.

NCBI and Uniprot Product Information

• NCBI GI #: 205830665
• NCBI GeneID: 4684
• UniProt Accession #: P13591; P13592
• Molecular Weight: 180, 145 and 125kDa
• NCBI Official Full Name: Neural cell adhesion molecule 1
• NCBI Official Synonym Full Names: neural cell adhesion molecule 1
• NCBI Official Symbol: NCAM1
• NCBI Official Synonym Symbols: CD56; NCAM; MSK39
• NCBI Protein Information: neural cell adhesion molecule 1
• UniProt Protein Name: Neural cell adhesion molecule 1
• Protein Family: Neural cell adhesion molecule
• UniProt Gene Name: NCAM1
• UniProt Synonym Gene Names: NCAM; N-CAM-1; NCAM-1
• UniProt Entry Name: NCAM1_HUMAN

NCBI Description

This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. The encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2011]

Uniprot Description

NCAM1: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, GPI anchor; Membrane protein, integral; Cell adhesion; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: Golgi membrane; cell surface; membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function: identical protein binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; cytokine and chemokine mediated signaling pathway; cell adhesion