Mouse anti-Rat CD5 Monoclonal Antibody | anti-CD5 antibody

Anti-CD5 Antibody, Mouse Monoclonal

Cat. Num. AAA8112981

• Reactivity: Rat
• Applications: Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: Protein A
• Synonyms: CD5; Monoclonal Antibody; Anti-CD5 Antibody; Mouse Monoclonal; CD5/Cluster of Differentiation 5 Antibody; Mouse MAb; CD5 molecule; anti-CD5 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 06
• Specificity: Rat CD5/Cluster of Differentiation 5
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD5 antibody: Flow Cytometry (FC/FACS/FCM), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: ICC/IF: 1:20-1:100; FC/FACS/FCM: 1:25-1:100
• Immunogen: Recombinant Rat CD5/Cluster of Differentiation 5 Protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD5/Cluster of Differentiation 5 (rR CD5/Cluster of Differentiation 5). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Rat CD5 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Immunofluorescence (IF)

(Immunofluorescence staining of ratCD5 in rat spleen cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti- ratCD5 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to Cell membrane.)

Related Product Information for anti-CD5 antibody

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

Product Categories/Family for anti-CD5 antibody

ITIM/ITAM Immunoreceptors and Related Molecules

References

Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12.Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.

NCBI and Uniprot Product Information

• NCBI GI #: 9506471
• NCBI GeneID: 54236
• NCBI Accession #: NP_062168.1
• NCBI GenBank Nucleotide #: NM_019295.1
• UniProt Accession #: P51882; Q63098
• Molecular Weight: 53,440 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD5
• NCBI Official Synonym Full Names: Cd5 molecule
• NCBI Official Symbol: Cd5
• NCBI Protein Information: T-cell surface glycoprotein CD5; CD5 antigen; Lymphocyte antigen CD5
• UniProt Protein Name: T-cell surface glycoprotein CD5
• Protein Family: CD5 antigen
• UniProt Gene Name: Cd5
• UniProt Entry Name: CD5_RAT