Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD5 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD5, then a 488-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Mouse anti-Rat CD5 Monoclonal Antibody | anti-CD5 antibody
Anti-CD5 Antibody, Mouse Monoclonal
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD5; Monoclonal Antibody; Anti-CD5 Antibody; Mouse Monoclonal; CD5/Cluster of Differentiation 5 Antibody; Mouse MAb; CD5 molecule; anti-CD5 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
01
Specificity
Rat CD5/Cluster of Differentiation 5
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CD5 antibody
Flow Cytometry (FC/FACS/FCM)
Application Notes
FC/FACS/FCM: 1:25-1:100
Immunogen
Recombinant Rat CD5/Cluster of Differentiation 5 Protein
Conjugation
Unconjugated
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rat CD5/Cluster of Differentiation 5 (rR CD5/Cluster of Differentiation 5). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD5 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD5, then a 488-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Rat CD5 expression on SD rat splenocytes. Cells were stained with purified anti-Rat CD5, then a 488-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-CD5 antibody
The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.
Product Categories/Family for anti-CD5 antibody
References
Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12.Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,440 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
Cd5 molecule
NCBI Official Symbol
Cd5
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen; Lymphocyte antigen CD5
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
Cd5
UniProt Entry Name
CD5_RAT
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Product Notes
The CD5 cd5 (Catalog #AAA8112978) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD5 Antibody, Mouse Monoclonal reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). FC/FACS/FCM: 1:25-1:100. Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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