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Testing Data (Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC)

Mouse CD4a Monoclonal Antibody | anti-CD4a antibody

CD4a (APC)

Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified by protein A affinity chromatography from tissue culture supernatant.
Synonyms
CD4a; Monoclonal Antibody; CD4a (APC); anti-CD4a antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
MIL17
Specificity
Recognizes porcine CD4a.
Purity/Purification
Purified by protein A affinity chromatography from tissue culture supernatant.
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-CD4a antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Immunogen
Leukocytes isolated from porcine gut lamina propria.
Conjugate
APC
Preparation and Storage
Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC)

Testing Data (Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD4a:FITC)

Testing Data

(Staining of porcine peripheral blood lymphocytes with.)

Testing Data (Staining of porcine peripheral blood lymphocytes with.)
Product Categories/Family for anti-CD4a antibody
References
1. Saalmu:ller, A. et al. (2001) Summary of workshop findings for porcine T-lymphocyte - specific monoclonal antibodies. Vet. Immunol. Immunopathol. 80: 35-52. 2. Castellano, G. et al. (2010) Therapeutic targeting of classical and lectin pathways of complement protects from ischemia-reperfusion-induced renal damage. Am J Pathol. 176: 1648-59. 3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa. J Leukoc Biol. Jun 15. 4. Kick, A.R. et al. (2010) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae. Res Vet Sci. Dec 11. 5. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs. J Anim Sci. 90: 649-56. 6. Goujon, J.M. et al. (2000) Influence of cold-storage conditions on renal function of autotransplanted large pig kidneys. Kidney Int. 58: 838-50. 7. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro. Cell Biol Int. 2012 Sep 13. 8. Tuchscherer, M. et al. (2012) Effects of inadequate maternal dietary protein:carbohydrate ratios during pregnancy on offspring immunity in pigs. BMC Veterinary Research 8: 232. 9. Cao, D. et al. (2010) Synthetic innate defence regulator peptide enhances in vivo immunostimulatory effects of CpG-ODN in newborn piglets. Vaccine. 28: 6006-13. 10. Clapperton, M. et al. (2005) Associations of weight gain and food intake with leukocyte sub-sets in Large White pigs Livestock Production Science 96: 249-60. 11. Clapperton, M. et al. (2005) Innate immune traits differ between Meishan and Large White pigs. Vet Immunol Immunopathol. 104: 131-44. 12. Clapperton, M. et al. (2008) Pig peripheral blood mononuclear leucocyte subsets are heritable and genetically correlated with performance. Animal. 2: 1575-84. 13. Faure, J.P. et al. (2002) Polyethylene glycol reduces early and long-term cold ischemia- reperfusion and renal medulla injury. J Pharmacol Exp Ther. 2002 Sep;302(3):861-70. 14. Faure, J.P. et al. (2004) Evidence for protective roles of polyethylene glycol plus high sodium solution and trimetazidine against consequences of renal medulla ischaemia during cold preservation and reperfusion in a pig kidney model. Nephrol Dial Transplant. 19: 1742-51. 15. Hauet, T. et al. (2002) Polyethylene glycol reduces the inflammatory injury due to cold ischemia/reperfusion in autotransplanted pig kidneys. Kidney Int. 62: 654-67. 16. Kick, A.R. et al. (2012) Effects of stress associated with weaning on the adaptive immune system in pigs. J Anim Sci. 90: 649-56. 17. Langerhuus, S.N. et al. (2010) Brief report: biomarkers of aortic vascular prosthetic graft infection in a porcine model with Staphylococcus aureus. Eur J Clin Microbiol Infect Dis. 29: 1453-6. 18. Lu, X. et al. (2012) Genome-wide association study for T lymphocyte subpopulations in swine. BMC Genomics. 13: 488. 19. Monroy-Salazar, H.G. et al. (2012) Effects of a live yeast dietary supplement on fecal coliform counts and on peripheral blood CD4+ and CD8+ lymphocyte subpopulations in nursery pigs. J Swine Health Prod 20: 276-282. 20. Shi, K. et al. (2008) Changes in peripheral blood leukocyte subpopulations in piglets co-infected experimentally with porcine reproductive and respiratory syndrome virus and porcine circovirus type 2. Vet Microbiol. 129: 367-77. 21. Spreeuwenberg, M.A. et al. (2001) Small intestine epithelial barrier function is compromised in pigs with low feed intake at weaning. J Nutr. 131: 1520-7. 22. Tambuyzer, B.R. et al. (2012) Osteopontin alters the functional profile of porcine microglia in vitro. Cell Biol Int. 36: 1233-8. 23. Zelnickova, P. et al. (2007) Intracellular cytokine detection by flow cytometry in pigs: fixation, permeabilization and cell surface staining. J Immunol Methods. 327: 18-29. 24. Kvist, P.H. et al. (2010) Effect of subcutaneous glucose sensor implantation on skin mRNA expression in pigs. Diabetes Technol Ther. 12: 791-9. 25. Lefevre, E.A. et al. (2012) Immune responses in pigs vaccinated with adjuvanted and non-adjuvanted A(H1N1)pdm/09 influenza vaccines used in human immunization programmes. PLoS One. 7(3): e32400. 26. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen- presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire. PLoS One. 7(3): e33707.

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Product Notes

The CD4a (Catalog #AAA6268555) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD4a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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