Rat CD49f Monoclonal Antibody | anti-CD49f antibody

CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (MaxLight 650)

Cat. Num. AAA6255722

• Reactivity: Canine, Human, Monkey, Mouse, Porcine, Sheep
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CD49f; Monoclonal Antibody; CD49f (CD49f Antigen; Integrin alpha 6; ITGA6; ITGA6B; VLA 6 alpha Subunit; VLA-6) (MaxLight 650); Integrin alpha chain; anti-CD49f antibody

• Host: Rat
• Reactivity: Canine, Human, Monkey, Mouse, Porcine, Sheep
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3H1515
• Specificity: Recognizes human CD49f. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). Reported to inhibit platelet adhesion to laminin. Species Crossreactivity: Mouse, canine, porcine, sheep, cynomolgus monkey.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight650.

• Applicable Applications for anti-CD49f antibody: Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Immunogen: BALB/c mouse mammary tumor cells.
• Conjugate: MaxLight650
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of human peripheral blood platelets with RAT ANTI HUMAN CD49f:Azide Free (MCA699XZ))

Testing Data

(Staining of human peripheral blood platelets with RAT ANTI HUMAN CD49f:Biotin (MCA699B))

Product Categories/Family for anti-CD49f antibody

Antibodies; CD Markers

References

1. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W. et al. Oxford University Press p1029-1032, 2. Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055. Oxford University Press, 3. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. Histochem. Cytochem. 34: 1037-1046, 4. Sonnenberg, A. et al. (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody J. Biol. Chem. 262: 10376-10383, 5. Helmer, M. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures J. Biol. Chem. 263: 7660-7665, 6. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes. Vet. Immunol. Immunopathol. 53: 329 - 334, 7. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6. Nature. 336: 487 - 489, 8. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragments E8. J. Cell. Biol. 110: 2145 - 2155, 9. Sonnenberg, A., et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumour cells using a monoclonal antibody against platelet glycoprotein Ic. J. Biol. Chem. 263: 14030 - 14038, 10. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim.49 (2): 97-110, 11. Sonneberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J. Cell Sci. 96: 207 - 217.