Mouse anti-Human CD48 Monoclonal Antibody | anti-CD48 antibody

anti-human CD48-Purified Preservative Free

Cat. Num. AAA666122

• Gene Names: CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Purified Preservative Free
• Synonyms: CD48; Monoclonal Antibody; anti-human CD48-Purified Preservative Free; Monoclonal anti-human CD48(Blast-1); anti-CD48 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 5-4.8
• Purity/Purification: Purified Preservative Free
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 252

• Applicable Applications for anti-CD48 antibody: Flow Cytometry (FC/FACS)
• Performance: Five x 105 cultured human Raji cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD48 antibody at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.87 log10 fluorescent units when compared to a Mouse IgG2a/FITC negative control at a similar concentration.
• Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
• Immunogen: Human PBLs
• Preparation and Storage: Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD48 antibody

Information: Human CD48 is a GPI anchored cell surface gylcoprotein found on all lymphocytes and monocytes. CD48 is a ligand for CD2 and may be involved in enhancing the activating signal to B cells. Antibody 5-4.8 recognizes epitope 4 of the CD48 molecule of 47 kd (4).

References

(1) H.A. Vaughan, et al, (1983) Transplantation 36: 446-450. (2) H.A. Vaughan, et al, (1991) Immunogenetics 33: 113-117. (3)E.N. Klyushnenkova, et al, (1996) Cellular Immunol 174: 90-98. (4)Leukocyte Typing VI (T. Kishimoto, et al, eds.) Garland Publishing, Inc., New York (1997) p. 509-512.

NCBI and Uniprot Product Information

• NCBI GI #: 365733590
• NCBI GeneID: 962
• NCBI Accession #: NP_001242959.1
• NCBI GenBank Nucleotide #: NM_001256030.1
• Molecular Weight: 19,429 Da
• NCBI Official Full Name: CD48 antigen isoform 2
• NCBI Official Synonym Full Names: CD48 molecule
• NCBI Official Symbol: CD48
• NCBI Official Synonym Symbols: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• NCBI Protein Information: CD48 antigen; TCT.1; BCM1 surface antigen; SLAM family member 2; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); signaling lymphocytic activation molecule 2
• UniProt Protein Name: CD48 antigen
• Protein Family: CD48 antigen
• UniProt Gene Name: CD48
• UniProt Synonym Gene Names: BCM1; BLAST1; SLAMF2
• UniProt Entry Name: CD48_HUMAN

NCBI Description

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

CD48: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 1q21.3-q22

Cellular Component: membrane; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein binding; receptor activity

Biological Process: defense response; blood coagulation; leukocyte migration