Mouse CD47 Monoclonal Antibody | anti-CD47 antibody

CD47 (AP)

Cat. Num. AAA6247676

• Gene Names: CD47; IAP; OA3; MER6
• Reactivity: Bovine, Canine, Human, Porcine, Sheep
• Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by protein G affinity chromatography from tissue culture supernatant.
• Synonyms: CD47; Monoclonal Antibody; CD47 (AP); anti-CD47 antibody

• Host: Mouse
• Reactivity: Bovine, Canine, Human, Porcine, Sheep
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 3H1456
• Specificity: Recognizes the human CD47. Species Crossreactivity: porcine, canine, sheep, bovine
• Purity/Purification: Purified by protein G affinity chromatography from tissue culture supernatant.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase.

• Applicable Applications for anti-CD47 antibody: Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Immunogen: Human erythrocytes
• Conjugate: AP
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Product Categories/Family for anti-CD47 antibody

Antibodies; CD Markers

References

Salajegheh, M. et al., (2007) J Neuroimmunol. 187:166-174. 1. Avent, N. et al. (1988) Monoclonal antibodies that recognize different membrane proteins that are deficient in Rhnull human erythrocytes. One group of antibodies reacts with a variety of cells and tissues whereas the other group is erythroid-specific. Biochem J. 251 (2): 499-505. 2. Campbell, I.G. et al. (1992) An ovarian tumor marker with homology to vaccinia virus contains an IgV-like region and multiple transmembrane domains. Cancer Res. 52 (19): 5416-20. 3. Brown, E. et al. (1990) Integrin-associated protein: a 50-kD plasma membrane antigen physically and functionally associated with integrins. J Cell Biol. 111 (6 Pt 1): 2785-94. 4. Lindberg, F.P. et al. (1993) Molecular cloning of integrin-associated protein: an immunoglobulin family member with multiple membrane-spanning domains implicated in alpha v beta 3-dependent ligand binding. J Cell Biol. 123 (2): 485-96. 5. Ide, K. et al. (2007) Role for CD47-SIRPalpha signaling in xenograft rejection by macrophages. Proc Natl Acad Sci U S A. 104 (12): 5062-6. 6. Lecchi, C. et al. (2008) Bovine alpha-1 acid glycoprotein can reduce the chemotaxis of bovine monocytes and modulate CD18 expression. Vet Res. 39: 50. 7. Chao, M.P. et al. (2011) Therapeutic antibody targeting of CD47 eliminates human acute lymphoblastic leukemia. Cancer Res. 71 (4): 1374-84. 8. Shahein, Y.E. et al. (2002) Molecular cloning and functional characterization of the pig homologue of integrin-associated protein (IAP/CD47). Immunology. 106: 564-76. 9. Brooke, G. et al. (2004) Human lymphocytes interact directly with CD47 through a novel member of the signal regulatory protein (SIRP) family. J Immunol. 173: 2562-70. 10. Wewer, C. et al. (2011) Transcellular migration of neutrophil granulocytes through the blood-cerebrospinal fluid barrier after infection with Streptococcus suis. J Neuroinflammation. 8: 51. 11. Ticchioni, M. et al. (2001) Integrin-associated protein (CD47/IAP) contributes to T cell arrest on inflammatory vascular endothelium under flow. FASEB J. 15: 341-50. 12. Chao, M.P. et al. (2010) Anti-CD47 antibody synergizes with rituximab to promote phagocytosis and eradicate non-Hodgkin lymphoma. Cell. 142: 699-713. 13. Siddhartha, J et al. (2016) Methods of manipulating phagocytosis mediated by CD47. US patent app 15/054930

NCBI and Uniprot Product Information

• NCBI GI #: 1171879
• NCBI GeneID: 961
• UniProt Accession #: Q08722; Q53Y71; Q96A60; A8K198; D3DN59
• Molecular Weight: 323
• NCBI Official Full Name: Leukocyte surface antigen CD47
• NCBI Official Synonym Full Names: CD47 molecule
• NCBI Official Symbol: CD47
• NCBI Official Synonym Symbols: IAP; OA3; MER6
• NCBI Protein Information: leukocyte surface antigen CD47; CD47 glycoprotein; Rh-related antigen; integrin associated protein; integrin-associated protein; integrin-associated signal transducer; antigenic surface determinant protein OA3; antigen identified by monoclonal antibody 1D
• UniProt Protein Name: Leukocyte surface antigen CD47
• Protein Family: Leukocyte surface antigen
• UniProt Gene Name: CD47
• UniProt Synonym Gene Names: MER6; IAP
• UniProt Entry Name: CD47_HUMAN

NCBI Description

This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

CD47: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q13.1-q13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: integrin-mediated signaling pathway; extracellular matrix organization and biogenesis; response to bacterium; positive regulation of phagocytosis; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; positive regulation of T cell activation; opsonization; blood coagulation; cell adhesion; leukocyte migration; positive regulation of inflammatory response