Mouse anti-Rat CD46 Monoclonal Antibody | anti-CD46 antibody

CD46, Rat, mAb MM1

Cat. Num. AAA584438

• Gene Names: CD46; MCP; TLX; AHUS2; MIC10; TRA2.10
• Reactivity: Rat
• Applications: Immunofluorescence, Western Blot
• Synonyms: CD46; Monoclonal Antibody; Rat; mAb MM1; Membrane cofactor protein; anti-CD46 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Form/Format: 1 ml (100 ug/ml) 0.2 um filtered antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide.; For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
• Sequence Length: 349

• Applicable Applications for anti-CD46 antibody: F, Immunoassay (IA), Immunofluorescence (IF), Western Blot (WB)
• Application Notes: Application Use: For immunohistology, immunofluorescence and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
• Immunogen: Second and third SCR domains of MCP fused to IgG-Fc; MCP23-Ig
• Positive Control: Western blotting: rat testis lysate, non-reducing conditions, band size 38 kDa (Ref. 1and 4)
• Preparation and Storage: Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.

Related Product Information for anti-CD46 antibody

Product Description: The monoclonal antibody MM.1 recognizes CD46, also known as membrane cofactor protein. An immunohistological survey of rat tissues revealed that CD46 is expressed only in the testis and epididymis of adult rats more specifically, only by spermatozoa and late spermatozoal precursors. In contrast, in humans, CD46 is expressed on every cell and tissue, with the exception of erythrocytes. CD46 serves to down-regulate the activation of complement on host tissue. It functions as a cofactor by binding to C3b and C4b. Binding is permitted by factor I, a serine protease of plasma, to degrade C3b and C4b and serves to protect the host cell against autologous attack. In human, it also serves as a receptor for measles virus. Two isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. The isoforms differ in the length. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)).

References

1. Mizuno, M et al. Rat membrane cofactor protein (MCP; CD46) is expressed only in the acrosome of developing and mature spermatozoa and mediates binding to immobilized activated C3. Biol reprod 2004, 71: 1374 2. Mizuno, M et al. Expression of CD46 in developing rat spermatozoa: ultrastructural localization and utility as a marker of the various stages of the seminiferous tubuli. Biol reprod 2005, 72: 908
3. Mizuno, M et al. Spermatogenic cells distal to the blood-testis barrier in rats lack C3 convertase regulators and may be at risk of complement-mediated injury. J Reprod Immunol 2006, 69: 23
4. Mizuno, M et al. CD55 in rat male reproductive tissue: differential expression in testis and expression of a unique truncated isoform on spermatozoa. Mol Immunol 2007, 44: 1613

NCBI and Uniprot Product Information

• NCBI GI #: 1256701
• NCBI GeneID: 4179
• Molecular Weight: 36,826 Da
• NCBI Official Full Name: CD46
• NCBI Official Synonym Full Names: CD46 molecule, complement regulatory protein
• NCBI Official Symbol: CD46
• NCBI Official Synonym Symbols: MCP; TLX; AHUS2; MIC10; TRA2.10
• NCBI Protein Information: membrane cofactor protein; measles virus receptor; complement membrane cofactor protein; trophoblast leucocyte common antigen; trophoblast leukocyte common antigen; CD46 antigen, complement regulatory protein; trophoblast-lymphocyte cross-reactive antigen; antigen identified by monoclonal antibody TRA-2-10; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen)
• UniProt Protein Name: Membrane cofactor protein
• Protein Family: Membrane cofactor protein
• UniProt Gene Name: CD46
• UniProt Synonym Gene Names: MCP; MIC10
• UniProt Entry Name: MCP_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]

Uniprot Description

CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Cell surface

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: Golgi apparatus; inner acrosomal membrane; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; plasma membrane

Molecular Function: enzyme inhibitor activity; complement binding; protein binding; cadherin binding; endopeptidase activity; receptor activity

Biological Process: positive regulation of memory T cell differentiation; adaptive immune response; positive regulation of regulatory T cell differentiation; viral reproduction; T cell mediated immunity; proteolysis; positive regulation of interleukin-10 production; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; negative regulation of catalytic activity; interleukin-10 production; regulation of complement activation; innate immune response; positive regulation of T cell proliferation; complement activation, classical pathway