Mouse anti-Human CD45RO Monoclonal Antibody | anti-CD45RO antibody

CD45RO (Leukocyte Common Antigen, LCA) (Biotin)

Cat. Num. AAA6122173

• Gene Names: PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CD45RO; Monoclonal Antibody; CD45RO (Leukocyte Common Antigen; LCA) (Biotin); anti-CD45RO antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: UCHL-1
• Specificity: Recognizes human CD45RO. Species Crossreactivity: chimpanzee and common marmoset.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
• Sequence Length: 87

• Applicable Applications for anti-CD45RO antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IP: 1-2ug/500ug protein lysate; FC/FACS: 0.5-2ug/10e6 cells; IF: 1-2ug/ml; WB: 0.5-1ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Interleukin-2-dependent human T lymphocytes
• Conjugate: Biotin
• Positive Control: CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human tonsil stained with CD45RO antibody (1:500), peroxidase-conjugate and DAB chromogen. Note cell membrane staining.)

Product Categories/Family for anti-CD45RO antibody

Antibodies; CD Markers

References

1. Streuli M, Morimoto C, Schrieber M, Schlossman S, Saito H. J Immunol. 141:3910-3914(1988). 2. Tonks N, Charbonneau H, Diltz C, Fischer E and Walsh K. Biochem. 27:8695-8701(1988). 3. Ledbetter JA, Tonks NA, Fischer EH, Clark EA. Proc Natl Acad Sci USA 85:8628-8632(1988). 4. Knapp W, et al. Eds. Leucocyte Typing IV. Oxford University Press. New York (1989). 5. Thomas M. Annu. Rev. Immunol. 7:339 (1989). 6. Picker LJ, Treer JR, Ferguson-Darnell B, Collins PA, Buck D, Terstappen L. J Immunol. 150:1105-1121(1993). 7. Trowbridge I, et al. Annu. Rev. Immunol. 12:85(1994). 8. Yamada M, et al. Stroke 27:1155 (1996). 9. Sakkas LI, et al. Clin. Diagn. Lab. Immunol. 5:430 (1998). 10. Ponsford M, et al. Clin. Exp. Immunol. 124:315 (2001). 11. D'Arena G, Cascavilla N, Nunziata G, et al. Leuk Lymphoma.43:1823-1825(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 392306955
• NCBI GeneID: 5788
• NCBI Accession #: NP_001254727
• NCBI GenBank Nucleotide #: NP_001254727.1
• UniProt Accession #: P08575
• NCBI Official Full Name: receptor-type tyrosine-protein phosphatase C isoform 5
• NCBI Official Synonym Full Names: protein tyrosine phosphatase receptor type C
• NCBI Official Symbol: PTPRC
• NCBI Official Synonym Symbols: LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180
• NCBI Protein Information: receptor-type tyrosine-protein phosphatase C
• UniProt Protein Name: Receptor-type tyrosine-protein phosphatase C
• UniProt Gene Name: PTPRC
• UniProt Synonym Gene Names: CD45; L-CA
• UniProt Entry Name: PTPRC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]

Uniprot Description

CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor protein phosphatase, tyrosine; EC 3.1.3.48

Chromosomal Location of Human Ortholog: 1q31-q32

Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding

Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; positive regulation of antigen receptor-mediated signaling pathway; T cell receptor signaling pathway; bone marrow development; B cell receptor signaling pathway; stem cell development; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; positive regulation of T cell proliferation; hemopoietic progenitor cell differentiation; negative regulation of cytokine and chemokine mediated signaling pathway; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation

Disease: Hepatitis C Virus, Susceptibility To; Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive