Mouse anti-Rat CD45RA Monoclonal Antibody | anti-CD45RA antibody
CD45RA (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE)
Reactivity
Rat
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified by Protein G Affinity Chromatography
Synonyms
CD45RA; Monoclonal Antibody; CD45RA (Leukocyte Common Antigen; LCA; Ly-5 T200) (PE); anti-CD45RA antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H1431
Specificity
Recognizes a high molecular weight band of the leukocyte common antigen (LCA) in rat. Only labels B-cells among thoracic duct lymphocytes with little labeling in bone marrow and none on thymocytes.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-CD45RA antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Immunogen
Purified spleen leucocyte common antigen.
Conjugate
PE
Preparation and Storage
Store at 4 degree C. Do Not Freeze.
Product Categories/Family for anti-CD45RA antibody
References
1. Barclay AN and McCall MN. CD45; from alloantigen to mapping of restricted epitopes using recombinant soluble CD45 isoforms. Biochem. Soc. Trans. 1992 20:161 PubMed 2. Trowbridge IS and Thomas ML. CD45: an emerging role as a protein tyrosine phosphatase required for lymphocyte activation and development. Annu. Rev. Immunol. 1993 12:85 PubMed 3. Beverley PCL. 1980. in Production and Use of Monoclonal Antibodies in Transplantation Immunology. In Transplantation and Clinical Immunology XI. pp 87-94, eds Touraine JL et al, Excerpta Medica Amsterdam. 4. Kishihara K, Penninger J, Wallace VA, Kundig TM, Kawai K, Wakeham A, Timms E, Pfeffer K, Ohashi PS, Thomas ML and et al. Normal B lymphocyte development but impaired T cell maturation in CD45-exon6 protein tyrosine phosphatase-deficient mice. Cell 1993 74:143 PubMed 5. Morimoto C, Letvin NL, Distaso JA, Aldrich WR and Schlossman SF. The isolation and characterization of the human suppressor inducer T cell subset. J. Immunol. 1985 134:1508 PubMed 6. Okumura M, Matthews RJ, Robb B, Litman GW, Bork P and Thomas ML. Comparison of CD45 extracellular domain sequences from divergent vertebrate species suggests the conservation of three fibronectin type III domains. J. Immunol. 1996 157:1569 PubMed 7. Perillo NL, Pace KE, Seilhamer JJ and Baum LG. Apoptosis of T cells mediated by galectin-1. Nature 1995 378:736 PubMed 8. Pingel JT and Thomas ML. Evidence that the leukocyte-common antigen is required for antigen-induced T lymphocyte proliferation. Cell 1989 58:1055 PubMed 9. Pulido R, Cebrian M, Acevedo A, de Landazuri MO and Sanchez-Madrid F. Comparative biochemical and tissue distribution study of four distinct CD45 antigen specificities. J. Immunol. 1988 140:3851 PubMed 10. Roach T, Slater S, Koval M, White L, McFarland EC, Okumura M, Thomas M and Brown E. CD45 regulates Src family member kinase activity associated with macrophage integrin-mediated adhesion. Curr. Biol. 1997 7:408 PubMed 11. Terry LA, Brown MH and Beverley PC. The monoclonal antibody, UCHL1, recognizes a 180,000 MW component of the human leucocyte-common antigen, CD45. Immunology 1988 64:331PubMed 12. Tonks NK, Charbonneau H, Diltz CD, Fischer EH and Walsh KA. Demonstration that the leukocyte common antigen CD45 is a protein tyrosine phosphatase. Biochemistry 1988 27:8695 PubMed 13. Zapata JM, Pulido R, Acevedo A, Sanchez-Madrid F and de Landazuri MO. Human CD45RC specificity. A novel marker for T cells at different maturation and activation stages. J. Immunol. 1994 152:3852 PubMed
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Product Notes
The CD45RA (Catalog #AAA6251590) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD45RA (Leukocyte Common Antigen, LCA, Ly-5 T200) (PE) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD45RA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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